WB
Western blot analysis of Caveolin-3/CAV3 using anti-Caveolin-3/CAV3 antibody. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human placenta tissue lysates, Lane 2: human U-87MG whole cell lysates, Lane 3: human Hela whole cell lysates, Lane 4: rat stomach tissue lysates, Lane 5: rat testis tissue lysates, Lane 6: mouse stomach tissue lysates, Lane 7: mouse testis tissue lysates. After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-Caveolin-3/CAV3 antigen affinity purified polyclonal antibody at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using ECL Plus Western Blotting Substrate.IHC
IHC analysis of Caveolin-3/CAV3 using anti-Caveolin-3/CAV3 antibody. Caveolin-3/CAV3 was detected in a paraffin-embedded section of human skeletal muscle tissue. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.IF-P
IF analysis of Caveolin-3/CAV3 using anti-Caveolin-3/CAV3 antibody. Caveolin-3/CAV3 was detected in a paraffin-embedded section of human cardiac muscle tissue. Cy3-conjugated Anti-rabbit IgG Secondary Antibody (red) was used as secondary antibody. The section was counterstained with DAPI (Blue).IF-P
IF analysis of Caveolin-3/CAV3 using anti-Caveolin-3/CAV3 antibody. Caveolin-3/CAV3 was detected in a paraffin-embedded section of mouse cardiac muscle tissue. Cy3-conjugated Anti-rabbit IgG Secondary Antibody (red) was used as secondary antibody. The section was counterstained with DAPI(Blue).IF-P
IF analysis of Caveolin-3/CAV3 using anti-Caveolin-3/CAV3 antibody. Caveolin-3/CAV3 was detected in a paraffin-embedded section of rat cardiac muscle tissue. Cy3-conjugated Anti-rabbit IgG Secondary Antibody (red) was used as secondary antibody. The section was counterstained with DAPI (Blue).| Product Name | Anti-Caveolin-3/CAV3 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | E.coli-derived human Caveolin-3/CAV3 recombinant protein (Position: M1-D55). |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IF-PIHCWB |
| WB:1:500-2000 IHC:1:50-400 IF-P:1:50-400 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | CAV3 |
|---|---|
| Gene Synonyms | LQT9 MPDT RMD2 VIP21 LGMD1C VIP-21 |
| Gene Full Name | caveolin 3 |
| Gene Summary | This gene encodes a caveolin family member, which functions as a component of the caveolae plasma membranes found in most cell types. Caveolin proteins are proposed to be scaffolding proteins for organizing and concentrating certain caveolin-interacting molecules. Mutations identified in this gene lead to interference with protein oligomerization or intra-cellular routing, disrupting caveolae formation and resulting in Limb-Girdle muscular dystrophy type-1C (LGMD-1C), hyperCKemia or rippling muscle disease (RMD). Alternative splicing has been identified for this locus, with inclusion or exclusion of a differentially spliced intron. In addition, transcripts utilize multiple polyA sites and contain two potential translation initiation sites. [provided by RefSeq, Jul 2008] |
| Alternative Names | CAV3 caveolin 3 Caveolin-3 LGMD1C LQT9 M caveolin VIP 21 VIP21 |
| Molecular Weight(MW) | 17kDa(Observed MW 17kDa) |
| Cellular Localization | Cell membrane |
WB
Western blot analysis of Caveolin-3/CAV3 using anti-Caveolin-3/CAV3 antibody. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human placenta tissue lysates, Lane 2: human U-87MG whole cell lysates, Lane 3: human Hela whole cell lysates, Lane 4: rat stomach tissue lysates, Lane 5: rat testis tissue lysates, Lane 6: mouse stomach tissue lysates, Lane 7: mouse testis tissue lysates. After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-Caveolin-3/CAV3 antigen affinity purified polyclonal antibody at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using ECL Plus Western Blotting Substrate.
IHC
IHC analysis of Caveolin-3/CAV3 using anti-Caveolin-3/CAV3 antibody. Caveolin-3/CAV3 was detected in a paraffin-embedded section of human skeletal muscle tissue. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.
IF-P
IF analysis of Caveolin-3/CAV3 using anti-Caveolin-3/CAV3 antibody. Caveolin-3/CAV3 was detected in a paraffin-embedded section of human cardiac muscle tissue. Cy3-conjugated Anti-rabbit IgG Secondary Antibody (red) was used as secondary antibody. The section was counterstained with DAPI (Blue).
IF-P
IF analysis of Caveolin-3/CAV3 using anti-Caveolin-3/CAV3 antibody. Caveolin-3/CAV3 was detected in a paraffin-embedded section of mouse cardiac muscle tissue. Cy3-conjugated Anti-rabbit IgG Secondary Antibody (red) was used as secondary antibody. The section was counterstained with DAPI(Blue).
IF-P
IF analysis of Caveolin-3/CAV3 using anti-Caveolin-3/CAV3 antibody. Caveolin-3/CAV3 was detected in a paraffin-embedded section of rat cardiac muscle tissue. Cy3-conjugated Anti-rabbit IgG Secondary Antibody (red) was used as secondary antibody. The section was counterstained with DAPI (Blue).| Application Notes | WB:1:500-2000 IHC:1:50-400 IF-P:1:50-400 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | 12 months from date of receipt，-20℃ as supplied. |
| Storage Buffer | 500 ug/ml antibody with PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol. |
