WB
Western blot analysis of XCR1 using anti-XCR1 antibody. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: RT4 whole cell lysates, Lane 2: U-87MG whole cell lysates, Lane 3: HEL whole cell lysates, Lane 4: U2OS whole cell lysates, Lane 5: rat spleen tissue lysates, Lane 6: mouse spleen tissue lysates, Lane 7: mouse thymus tissue lysates. After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-XCR1 antigen affinity purified polyclonal antibody at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using ECL Plus Western Blotting Substrate.IHC
IHC analysis of XCR1 using anti-XCR1 antibody. XCR1 was detected in a paraffin-embedded section of mouse spleen tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-XCR1 Antibody at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.IHC
IHC analysis of XCR1 using anti-XCR1 antibody. XCR1 was detected in a paraffin-embedded section of mouse thymus tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-XCR1 Antibody at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.| Product Name | Anti-XCR1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | E. coli-derived human CCXCR1/XCR1 recombinant protein (Position: F246-N274). Human XCR shares 59.3% amino acid (aa) sequence identity with mouse XCR. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHCWB |
| WB:1:500-2000 IHC:1:50-400 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | XCR1 |
|---|---|
| Gene Synonyms | GPR5 CCXCR1 |
| Gene Full Name | X-C motif chemokine receptor 1 |
| Gene Summary | The protein encoded by this gene is a chemokine receptor belonging to the G protein-coupled receptor superfamily. The family members are characterized by the presence of 7 transmembrane domains. The encoded protein transduces a signal by increasing the intracellular calcium ion level. The viral macrophage inflammatory protein-II is an antagonist of this receptor and blocks signaling. Some studies have implicated a cluster of genes at 3p21.31, including this gene, as associated with COVID-19 risk. The encoded protein may also play a role in cell proliferation and migration in several types of cancer. [provided by RefSeq, Jan 2023] |
| Alternative Names | XCR1 CCXCR1 GPR5 Chemokine XC receptor 1 Lymphotactin receptor XC chemokine receptor 1 |
| Molecular Weight(MW) | 39kDa(Observed MW 42kDa) |
| Cellular Localization | Cell membrane |
WB
Western blot analysis of XCR1 using anti-XCR1 antibody. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: RT4 whole cell lysates, Lane 2: U-87MG whole cell lysates, Lane 3: HEL whole cell lysates, Lane 4: U2OS whole cell lysates, Lane 5: rat spleen tissue lysates, Lane 6: mouse spleen tissue lysates, Lane 7: mouse thymus tissue lysates. After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-XCR1 antigen affinity purified polyclonal antibody at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using ECL Plus Western Blotting Substrate.
IHC
IHC analysis of XCR1 using anti-XCR1 antibody. XCR1 was detected in a paraffin-embedded section of mouse spleen tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-XCR1 Antibody at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
IHC
IHC analysis of XCR1 using anti-XCR1 antibody. XCR1 was detected in a paraffin-embedded section of mouse thymus tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-XCR1 Antibody at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.| Application Notes | WB:1:500-2000 IHC:1:50-400 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | 12 months from date of receipt，-20℃ as supplied. |
| Storage Buffer | 500 ug/ml antibody with PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol. |
