WB
Western Blot, Positive WB detected in: 293F whole cell lysate, scFv-based Anti-CD19 CAR containing a Whitlow linker (293F/CD19 CAR) whole cell lysate, All lanes: Whitlow linker antibody at 1:1000, Secondary,Goat polyclonal to mouse IgG at 1/50000 dilution,Exposure time：5s.FC
Untransfected HEK-293F cells (green line) and transfected scFv-based Anti-CD19 CAR containing a Whitlow/218 linker stable cells (red line) were stained with anti-Whitlow linker antibody (2µg/1*106cells), washed and then followed by FITC-conjugated anti-Mouse IgG Fc antibody and analyzed with flow cytometry.ELISA
The Binding Activity of Whitlow linker with Anti-Whitlow linker antibody Activity: Measured by its binding ability in a functional ELISA. Immobilized scFv-based Anti-CD19 CAR recombinant antibody at 2 μg/mL can bind Anti-Whitlow linker antibody. The EC50 is 5.147 to 5.761 ng/mL.| Product Name | Whitlow linker Monoclonal Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Whitlow linker-KLH |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG2b |
| Host Species | Mouse |
| Tested Applications | ELISAFCWB |
| WB:1:1000-1:5000 FC:1:50-1:200 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Protein G |
| Gene Symbol | LAT |
|---|---|
| Gene Synonyms | LAT1 pp36 IMD52 |
| Gene Full Name | linker for activation of T cells |
| Gene Summary | The protein encoded by this gene is phosphorylated by ZAP-70/Syk protein tyrosine kinases following activation of the T-cell antigen receptor (TCR) signal transduction pathway. This transmembrane protein localizes to lipid rafts and acts as a docking site for SH2 domain-containing proteins. Upon phosphorylation, this protein recruits multiple adaptor proteins and downstream signaling molecules into multimolecular signaling complexes located near the site of TCR engagement. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008] |
| Alternative Names | Whitlow/218 Linker (E3U7Q) Whitlow linker |
| Molecular Weight(MW) | 50-200kDa(Observed MW 50-200kDa) |
WB
Western Blot, Positive WB detected in: 293F whole cell lysate, scFv-based Anti-CD19 CAR containing a Whitlow linker (293F/CD19 CAR) whole cell lysate, All lanes: Whitlow linker antibody at 1:1000, Secondary,Goat polyclonal to mouse IgG at 1/50000 dilution,Exposure time：5s.
FC
Untransfected HEK-293F cells (green line) and transfected scFv-based Anti-CD19 CAR containing a Whitlow/218 linker stable cells (red line) were stained with anti-Whitlow linker antibody (2µg/1*106cells), washed and then followed by FITC-conjugated anti-Mouse IgG Fc antibody and analyzed with flow cytometry.
ELISA
The Binding Activity of Whitlow linker with Anti-Whitlow linker antibody Activity: Measured by its binding ability in a functional ELISA. Immobilized scFv-based Anti-CD19 CAR recombinant antibody at 2 μg/mL can bind Anti-Whitlow linker antibody. The EC50 is 5.147 to 5.761 ng/mL.| Application Notes | WB:1:1000-1:5000 FC:1:50-1:200 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. |
| Storage Buffer | Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, PH 7.4 |
