WB
Western Blot, Positive WB detected in: HepG2 whole cell lysate, Hela whole cell lysate, PC-3 whole cell lysate, A549 whole cell lysate, All lanes PD-L2 antibody at 1:2000, Secondary, Goat polyclonal to mouse IgG at 1/50000 dilution, Exposure time:5min .WB
Western Blot, Positive WB detected in: Hela whole cell lysate at 20μg, 10μg, 5μg, 2.5μg, 1.25μg All lanes: PD-L2 antibody at 1:2000, Secondary, Goat polyclonal to mouse IgG at 1/50000 dilution, Exposure time:5min .WB
Western Blot, Positive WB detected in: 15μg hela whole cell lysate PD-L2 antibody at 1:5000, 1:10000, 1:20000, 1:40000, 1:80000, 1:160000, 1:320000, 1:640000, Secondary, Goat polyclonal to mouse IgG at 1/50000 dilution, Exposure time:5min.IHC
IHC image of PD-L2 Monoclonal Antibody diluted at 1:100 and staining in paraffin-embedded human tonsil tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.ICC/IF
Immunofluorescence staining of HepG2 cells with PD-L2 Monoclonal Antibody at 1:100, counter-stained with DAPI. The cells were incubated with the antibody overnight at 4°C. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was FITC-conjugated AffiniPure Goat Anti-Mouse IgG (H+L).ICC/IF
Immunofluorescence staining of Hela cells with PD-L2 Monoclonal Antibody at 1:100, counter-stained with DAPI. The cells were incubated with the antibody overnight at 4°C. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was FITC-conjugated AffiniPure Goat Anti-Mouse IgG (H+L).ICC/IF
Immunofluorescence staining of Raji cells with PD-L2 Monoclonal Antibody at 1:100, counter-stained with DAPI. The cells were incubated with the antibody overnight at 4°C. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was FITC-conjugated AffiniPure Goat Anti-Mouse IgG (H+L).ICC/IF
Immunofluorescence staining of U251 cells with PD-L2 Monoclonal Antibody at 1:100, counter-stained with DAPI. The cells were incubated with the antibody overnight at 4°C. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was FITC-conjugated AffiniPure Goat Anti-Mouse IgG (H+L).FC
Overlay histogram showing 293 cells transfected with PD-L2 stained with PD-L2 Monoclonal Antibody (red line). The cells were incubated in 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (2µg/1*106cells) for 1 h at 4°C. The secondary antibody used was R-PE-conjugated Goat Anti-Mouse IgG(H+L) at 1/100 dilution for 30min at 4°C. Isotype control antibody (green line) was mouse IgG2b (2µg/1*106cells) used under the same conditions. Acquisition of >10,000 events was performed.IP
Immunoprecipitating PD-L2 in Hela whole cell lysate, Lane 1: Mouse control IgG instead of PD-L2 Monoclonal Antibody in Hela whole cell lysate, Lane 2: PD-L2 Monoclonal Antibody (2µl) + Hela whole cell lysate (500µg), Lane 3: Hela whole cell lysate (20µg), For western blotting, the blot was detected with PD-L2 Monoclonal Antibody at 1:2000, and a HRP-conjugated Protein G antibody was used as the secondary antibody at 1:2000.| Product Name | PD-L2 Monoclonal Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant Human Programmed cell death 1 ligand 2 protein (21-118AA) |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG2b |
| Host Species | Mouse |
| Tested Applications | FCICC/IFIHCIPWB |
| WB:1:5000-1:640000 IHC:1:50-1:200 ICC/IF:1:50-1:200 FC:1:50-1:200 IP:2μl-5μl |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | PDCD1LG2 |
|---|---|
| Gene Synonyms | B7DC Btdc PDL2 CD273 PD-L2 PDCD1L2 bA574F11.2 |
| Gene Full Name | programmed cell death 1 ligand 2 |
| Gene Summary | Involved in negative regulation of activated T cell proliferation; negative regulation of interleukin-10 production; and negative regulation of type II interferon production. Predicted to be located in plasma membrane. Predicted to be active in external side of plasma membrane. Biomarker of pulmonary tuberculosis. [provided by Alliance of Genome Resources, Jul 2025] |
| Molecular Weight(MW) | 31/21kDa(Observed MW 45-50kDa) |
| Cellular Localization | Secreted |
WB
Western Blot, Positive WB detected in: HepG2 whole cell lysate, Hela whole cell lysate, PC-3 whole cell lysate, A549 whole cell lysate, All lanes PD-L2 antibody at 1:2000, Secondary, Goat polyclonal to mouse IgG at 1/50000 dilution, Exposure time:5min .
WB
Western Blot, Positive WB detected in: Hela whole cell lysate at 20μg, 10μg, 5μg, 2.5μg, 1.25μg All lanes: PD-L2 antibody at 1:2000, Secondary, Goat polyclonal to mouse IgG at 1/50000 dilution, Exposure time:5min .
WB
Western Blot, Positive WB detected in: 15μg hela whole cell lysate PD-L2 antibody at 1:5000, 1:10000, 1:20000, 1:40000, 1:80000, 1:160000, 1:320000, 1:640000, Secondary, Goat polyclonal to mouse IgG at 1/50000 dilution, Exposure time:5min.
IHC
IHC image of PD-L2 Monoclonal Antibody diluted at 1:100 and staining in paraffin-embedded human tonsil tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
ICC/IF
Immunofluorescence staining of HepG2 cells with PD-L2 Monoclonal Antibody at 1:100, counter-stained with DAPI. The cells were incubated with the antibody overnight at 4°C. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was FITC-conjugated AffiniPure Goat Anti-Mouse IgG (H+L).
ICC/IF
Immunofluorescence staining of Hela cells with PD-L2 Monoclonal Antibody at 1:100, counter-stained with DAPI. The cells were incubated with the antibody overnight at 4°C. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was FITC-conjugated AffiniPure Goat Anti-Mouse IgG (H+L).
ICC/IF
Immunofluorescence staining of Raji cells with PD-L2 Monoclonal Antibody at 1:100, counter-stained with DAPI. The cells were incubated with the antibody overnight at 4°C. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was FITC-conjugated AffiniPure Goat Anti-Mouse IgG (H+L).
ICC/IF
Immunofluorescence staining of U251 cells with PD-L2 Monoclonal Antibody at 1:100, counter-stained with DAPI. The cells were incubated with the antibody overnight at 4°C. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was FITC-conjugated AffiniPure Goat Anti-Mouse IgG (H+L).
FC
Overlay histogram showing 293 cells transfected with PD-L2 stained with PD-L2 Monoclonal Antibody (red line). The cells were incubated in 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (2µg/1*106cells) for 1 h at 4°C. The secondary antibody used was R-PE-conjugated Goat Anti-Mouse IgG(H+L) at 1/100 dilution for 30min at 4°C. Isotype control antibody (green line) was mouse IgG2b (2µg/1*106cells) used under the same conditions. Acquisition of >10,000 events was performed.
IP
Immunoprecipitating PD-L2 in Hela whole cell lysate, Lane 1: Mouse control IgG instead of PD-L2 Monoclonal Antibody in Hela whole cell lysate, Lane 2: PD-L2 Monoclonal Antibody (2µl) + Hela whole cell lysate (500µg), Lane 3: Hela whole cell lysate (20µg), For western blotting, the blot was detected with PD-L2 Monoclonal Antibody at 1:2000, and a HRP-conjugated Protein G antibody was used as the secondary antibody at 1:2000.| Application Notes | WB:1:5000-1:640000 IHC:1:50-1:200 ICC/IF:1:50-1:200 FC:1:50-1:200 IP:2μl-5μl |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. |
| Storage Buffer | Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, PH 7.4 |
