WB
Western Blot, Positive WB detected in: U937 whole cell lysate, K562 whole cell lysate, 293 whole cell lysate, A549 whole cell lysate, All lanes: BTN3A1 antibody at 1: 1000, Secondary, Goat polyclonal to rabbit IgG at 1/50000 dilution.ICC/IF
Immunofluorescence staining of Hela cell with BTN3A1 Antibody at 1:20, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).ICC/IF
Immunofluorescence staining of Hela cell with 5% goat serum, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).ICC/IF
Immunofluorescence staining of SH-SY5Y cell with BTN3A1 Antibody at 1:20, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).ICC/IF
Immunofluorescence staining of SH-SY5Y cell with 5% goat serum, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).| Product Name | BTN3A1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant Human Butyrophilin subfamily 3 member A1 protein |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFWB |
| WB:1:500-1:1000 ICC/IF:1:20-1:200 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | BTN3A1 |
|---|---|
| Gene Synonyms | BTF5 BT3.1 CD277 BTN3.1 |
| Gene Full Name | butyrophilin subfamily 3 member A1 |
| Gene Summary | The butyrophilin (BTN) genes are a group of major histocompatibility complex (MHC)-associated genes that encode type I membrane proteins with 2 extracellular immunoglobulin (Ig) domains and an intracellular B30.2 (PRYSPRY) domain. Three subfamilies of human BTN genes are located in the MHC class I region: the single-copy BTN1A1 gene (MIM 601610) and the BTN2 (e.g., BTN2A1; MIM 613590) and BTN3 (e.g., BNT3A1) genes, which have undergone tandem duplication, resulting in 3 copies of each (summary by Smith et al., 2010 [PubMed 20208008]).[supplied by OMIM, Nov 2010] |
| Molecular Weight(MW) | 40-58kDa(Observed MW 48kDa) |
| Cellular Localization | Cell membrane; Single-pass type I membrane protein. |
WB
Western Blot, Positive WB detected in: U937 whole cell lysate, K562 whole cell lysate, 293 whole cell lysate, A549 whole cell lysate, All lanes: BTN3A1 antibody at 1: 1000, Secondary, Goat polyclonal to rabbit IgG at 1/50000 dilution.
ICC/IF
Immunofluorescence staining of Hela cell with BTN3A1 Antibody at 1:20, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
ICC/IF
Immunofluorescence staining of Hela cell with 5% goat serum, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
ICC/IF
Immunofluorescence staining of SH-SY5Y cell with BTN3A1 Antibody at 1:20, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
ICC/IF
Immunofluorescence staining of SH-SY5Y cell with 5% goat serum, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).| Application Notes | WB:1:500-1:1000 ICC/IF:1:20-1:200 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. |
| Storage Buffer | Preservative: 0.02% sodium azide Constituents: PBS containing 50% glycerol pH 7.3 |
