WB
Western Blot, Positive WB detected in: 293 whole cell lysate, MCF7 whole cell lysate, K562 whole cell lysate, A549 whole cell lysate, Mouse Brain tissue lysate, Mouse Brain tissue lysate, All lanes: MCM9 antibody at 1:1000, Secondary, Goat polyclonal to rabbit IgG at 1/50000 dilution.ICC/IF
Immunofluorescence staining of MCF7 cell with MCM9 Antibody at 1:30, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).ICC/IF
Immunofluorescence staining of MCF7 cell with 5% goat serum, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).| Product Name | MCM9 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Synthesized peptide derived from part region of human protein. AA range 220-240 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFWB |
| WB:1:1000-1:5000 ICC/IF:1:20-1:200 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | MCM9 |
|---|---|
| Gene Synonyms | ODG4 MCMDC1 C6orf61 dJ329L24.1 dJ329L24.3 |
| Gene Full Name | minichromosome maintenance 9 homologous recombination repair factor |
| Gene Summary | The protein encoded by this gene is a member of the mini-chromosome maintenance (MCM) protein family that are essential for the initiation of eukaryotic genome replication. Binding of this protein to chromatin has been shown to be a pre-requisite for recruiting the MCM2-7 helicase to DNA replication origins. This protein also binds, and is a positive regulator of, the chromatin licensing and DNA replication factor 1, CDT1. [provided by RefSeq, Nov 2010] |
| Molecular Weight(MW) | 127/85/72/48kDa(Observed MW 127kDa) |
| Cellular Localization | Nucleus. Chromosome. |
WB
Western Blot, Positive WB detected in: 293 whole cell lysate, MCF7 whole cell lysate, K562 whole cell lysate, A549 whole cell lysate, Mouse Brain tissue lysate, Mouse Brain tissue lysate, All lanes: MCM9 antibody at 1:1000, Secondary, Goat polyclonal to rabbit IgG at 1/50000 dilution.
ICC/IF
Immunofluorescence staining of MCF7 cell with MCM9 Antibody at 1:30, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
ICC/IF
Immunofluorescence staining of MCF7 cell with 5% goat serum, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).| Application Notes | WB:1:1000-1:5000 ICC/IF:1:20-1:200 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. |
| Storage Buffer | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
