WB
Western blot with Anti-Phospho-REPS1(Ser709) antibody on lysates of mouse testis tissue (1) .12% SDS-PAGE gel.Sample loading: 20μg /lane. Transfer the proteins onto a PVDF membrane (OM790003), and block it with TBST (OM750016) containing 5% BSA (OM297470) for one hour. Dilute the primary antibody with the antibody diluent (OM750012) at a ratio of 1:1000, and incubate it overnight at 4°C. Wash the membrane three times with TBST (OM750016), 5 minutes each time. At room temperature, dilute the secondary antibody, Goat Anti-Rabbit IgG(H&L)-HRP (OM643487), at a ratio of 1:10000 and incubate for one hour. Wash the membrane three times with TBST (OM750016) again, 5 minutes each time. Use ECL (OM625701) for luminescence.staining time: 120S.| Product Name | Anti-Phospho-REPS1(Ser709) antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Synthetic peptide conjugated with KLH |
| Modification | p-Ser709 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:1000 |
|
| Species Reactivity | Mouse |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | REPS1 |
|---|---|
| Gene Synonyms | REPS1 |
| Gene Full Name | RalBP1-associated Eps domain-containing protein 1 |
| Gene Summary | EPS1 encodes a signaling adaptor protein that links protein interactions to endocytosis, endosomal transport, and cytoskeletal changes. The protein contains two EH domains and an EF-hand domain, placing it among adaptor proteins that coordinate signaling with membrane traffic. It is found in the clathrin-coated pit, plasma membrane, cytoplasm, and nucleoplasm, and its expression is high in brain and the proximal digestive tract. |
| Alternative Names | RALBP1 RalBP1 associated Eps domain containing protein 1 RalBP1 interacting protein 1 RalBP1-associated Eps domain-containing protein 1 RalBP1-interacting protein 1 REPS1 REPS1_HUMAN |
| Molecular Weight(MW) | 87kDa(Predicted); 125kDa(Observed） |
| Cellular Localization | Membrane, clathrin-coated pit |
| SwissProt ID | Q96D71 |
|---|
WB
Western blot with Anti-Phospho-REPS1(Ser709) antibody on lysates of mouse testis tissue (1) .12% SDS-PAGE gel.Sample loading: 20μg /lane. Transfer the proteins onto a PVDF membrane (OM790003), and block it with TBST (OM750016) containing 5% BSA (OM297470) for one hour. Dilute the primary antibody with the antibody diluent (OM750012) at a ratio of 1:1000, and incubate it overnight at 4°C. Wash the membrane three times with TBST (OM750016), 5 minutes each time. At room temperature, dilute the secondary antibody, Goat Anti-Rabbit IgG(H&L)-HRP (OM643487), at a ratio of 1:10000 and incubate for one hour. Wash the membrane three times with TBST (OM750016) again, 5 minutes each time. Use ECL (OM625701) for luminescence.staining time: 120S.| Application Notes | WB:1:1000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C long term. Avoid freeze / thaw cycle. |
| Storage Buffer | Purified antibody in PBS with 0.05% sodium azide,40% Glycerol. |
