ELISA
Microtiter plate wells were coated with 100 ng/well coating antigen and incubated with serial dilutions of the antibody. Following HRP-conjugated secondary antibody and substrate development, OD630 was measured. The absorbance decreased progressively with increasing antibody dilution, yielding a sigmoidal titration curve that demonstrates the high antigen-binding activity of the polyclonal antibody.WB
| Product Name | Anti-Phospho-Fyn(Tyr530)[Tyr531] antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Synthetic peptide conjugated with KLH |
| Modification | p-Tyr530/Tyr531 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAWB |
| WB:1:1000 |
|
| Species Reactivity | Mouse |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | FYN |
|---|---|
| Gene Synonyms | SLK SYN p59-FYN |
| Gene Full Name | FYN proto-oncogene, Src family tyrosine kinase |
| Gene Summary | This gene is a member of the protein-tyrosine kinase oncogene family. It encodes a membrane-associated tyrosine kinase that has been implicated in the control of cell growth. The protein associates with the p85 subunit of phosphatidylinositol 3-kinase and interacts with the fyn-binding protein. Alternatively spliced transcript variants encoding distinct isoforms exist. [provided by RefSeq, Jul 2008] |
| Alternative Names | C syn protooncogene Fyn FYN oncogene related to SRC FGR YES FYN_HUMAN OKT3 induced calcium influx regulator P59 FYN p59-Fyn Protein tyrosine kinase fyn Proto oncogene tyrosine protein kinase fyn Proto-oncogene c-Fyn Proto-oncogene Syn Protooncogene Syn SLK Src like kinase Src yes related novel gene Src-like kinase Src/yes related novel SYN Tyrosine kinase p59fyn T Tyrosine kinase p59fyn(T) Tyrosine-protein kinase Fyn |
| Molecular Weight(MW) | 61kDa |
| Cellular Localization | Cytoplasm. Nucleus. Cell membrane. |
| SwissProt ID | P06241 |
|---|
ELISA
Microtiter plate wells were coated with 100 ng/well coating antigen and incubated with serial dilutions of the antibody. Following HRP-conjugated secondary antibody and substrate development, OD630 was measured. The absorbance decreased progressively with increasing antibody dilution, yielding a sigmoidal titration curve that demonstrates the high antigen-binding activity of the polyclonal antibody.
WB
| Application Notes | WB:1:1000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C long term. Avoid freeze / thaw cycle. |
| Storage Buffer | Purified antibody in PBS with 0.05% sodium azide,40% Glycerol. |
