ELISA
Coat the plate with 100ng immunizing antigen. The phospho-specific antibody,pbs only can specifically recognize phosphorylated peptide and enable quantitative detection over a wide dynamic range. Non-phosphorylated peptide shows no cross-reactivity, and the background signal remains low, indicating that the phospho-specific antibody,pbs only possesses high specificity and an excellent signal-to-noise ratio. Black line: phospho-peptide; Rad line: non-phospho peptideDB
Dot blot analysis was performed using Anti-Phospho-JNK1/2/3 (T183 + T183 + T221) antibody to detect two peptide samples: lane 1 contains the phospho-peptide , and lane 2 contains the non-phospho-peptide. Transfer the proteins onto a NC membrane (OM790001), and block it with TBST (OM750016) containing 5% BSA(OM297470) for 1 hour. Dilute the primary antibody with the antibody diluent (OM750012) at a ratio of 1:1000, and incubate it at room temperature for 2 hours. Wash the membrane 3 times with TBST (OM750016), 5 minutes each time. At room temperature, dilute the secondary antibody, Goat Anti-Rabbit IgG(H&L)-HRP (OM643487), at a ratio of 1:10000 and incubate for 1 hour. Wash the membrane 3 times with TBST (OM750016) again, 5 minutes each time. Use ECL (OM625701) for luminescence. Exposure time: 30 s.| Product Name | Anti-Phospho-JNK1/2/3 (T183 + T183 + T221) Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Synthetic peptide conjugated with KLH. |
| Modification | p-T183 + T183 + T221 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | DBELISA |
| DB:1:1000 |
|
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | MAPK8/MAPK9/MAPK10 |
|---|---|
| Gene Synonyms | JNK JNK1 PRKM8 SAPK1 JNK-46 JNK1A2 SAPK1c JNK21B1/2JNK2 SAPK p54a JNK2A JNK2B PRKM9 JNK-55 SAPK1a JNK2BETA p54aSAPK JNK2ALPHA |
| Gene Full Name | mitogen-activated protein kinase 8/9/10 |
| Gene Summary | The protein encoded by this gene is a member of the MAP kinase family. MAP kinases act as an integration point for multiple biochemical signals, and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. This kinase is activated by various cell stimuli, and targets specific transcription factors, and thus mediates immediate-early gene expression in response to cell stimuli. The activation of this kinase by tumor-necrosis factor alpha (TNF-alpha) is found to be required for TNF-alpha induced apoptosis. This kinase is also involved in UV radiation induced apoptosis, which is thought to be related to cytochrom c-mediated cell death pathway. Studies of the mouse counterpart of this gene suggested that this kinase play a key role in T cell proliferation, apoptosis and differentiation. Several alternatively spliced transcript variants encoding distinct isoforms have been reported. [provided by RefSeq, Apr 2016] |
| Alternative Names | C Jun kinase 2 antibody c Jun N terminal kinase 1 antibody c Jun N terminal kinase 2 antibody c Jun N terminal kinase 3 antibody c-Jun N-terminal kinase 1 antibody JNK 46 antibody JNK 55 antibody JNK antibody JNK-46 antibody JNK1 antibody JNK1A2 antibody JNK2 antibody JNK21B1/2 antibody JNK2A antibody JNK2ALPHA antibody JNK2B antibody JNK2BETA antibody JNK3 alpha protein kinase antibody JNK3 antibody JNK3A antibody Jun kinase antibody JUN N terminal kinase antibody MAP kinase 10 antibody MAP kinase 8 antibody MAP kinase 9 antibody MAP kinase p49 3F12 antibody MAPK 10 antibody MAPK 8 antibody MAPK 9 antibody MAPK10 antibody mapk8 antibody MAPK9 antibody Mitogen activated protein kinase 10 antibody Mitogen activated protein kinase 8 antibody Mitogen activated protein kinase 8 isoform JNK1 alpha1 antibody Mitogen activated protein kinase 8 isoform JNK1 beta2 antibody Mitogen activated protein kinase 9 antibody Mitogen-activated protein kinase 8 antibody MK08_HUMAN antibody p493F12 antibody p54a antibody p54aSAPK antibody p54bSAPK antibody PRKM10 antibody PRKM8 antibody PRKM9 antibody SAPK antibody SAPK(beta) antibody SAPK1 antibody SAPK1a antibody SAPK1b antibody SAPK1c antibody Stress activated protein kinase 1 antibody Stress activated protein kinase 1a antibody Stress activated protein kinase 1b antibody Stress activated protein kinase 1c antibody Stress activated protein kinase beta antibody Stress activated protein kinase JNK1 antibody Stress activated protein kinase JNK2 antibody Stress activated protein kinase JNK3 antibody Stress-activated protein kinase 1c antibody Stress-activated protein kinase JNK1 antibody |
| Molecular Weight(MW) | 48/53 kDa |
| Cellular Localization | Cytoplasm, Membrane, Mitochondrion, Nucleus. |
| SwissProt ID | P45983/P45984/P53779 |
|---|
ELISA
Coat the plate with 100ng immunizing antigen. The phospho-specific antibody,pbs only can specifically recognize phosphorylated peptide and enable quantitative detection over a wide dynamic range. Non-phosphorylated peptide shows no cross-reactivity, and the background signal remains low, indicating that the phospho-specific antibody,pbs only possesses high specificity and an excellent signal-to-noise ratio. Black line: phospho-peptide; Rad line: non-phospho peptide
DB
Dot blot analysis was performed using Anti-Phospho-JNK1/2/3 (T183 + T183 + T221) antibody to detect two peptide samples: lane 1 contains the phospho-peptide , and lane 2 contains the non-phospho-peptide. Transfer the proteins onto a NC membrane (OM790001), and block it with TBST (OM750016) containing 5% BSA(OM297470) for 1 hour. Dilute the primary antibody with the antibody diluent (OM750012) at a ratio of 1:1000, and incubate it at room temperature for 2 hours. Wash the membrane 3 times with TBST (OM750016), 5 minutes each time. At room temperature, dilute the secondary antibody, Goat Anti-Rabbit IgG(H&L)-HRP (OM643487), at a ratio of 1:10000 and incubate for 1 hour. Wash the membrane 3 times with TBST (OM750016) again, 5 minutes each time. Use ECL (OM625701) for luminescence. Exposure time: 30 s.| Application Notes | DB:1:1000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C long term. Avoid freeze / thaw cycle. |
| Storage Buffer | Purified antibody in PBS with 0.05% sodium azide,40% Glycerol. |
