Western blot analysis in MDA-MB231 cell line lysates (35ug/lane).
CCNB1 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human uterus tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.
Western Blot at 1:1000 dilution + K562 whole cell lysate Lysates/proteins at 20 ug per lane.
The protein encoded by this gene is a regulatory protein
involved in mitosis. The gene product complexes with p34(cdc2) to
form the maturation-promoting factor (MPF). Two alternative
transcripts have been found, a constitutively expressed transcript
and a cell cycle-regulated transcript, that is expressed
predominantly during G2/M phase. The different transcripts result
from the use of alternate transcription initiation sites. [provided
by RefSeq].1) Kreis, N.N., et al. Oncogene 29(41):5591-5603(2010)
Immunogen
This CCNB1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 381-411 amino acids from the C-terminal region of human CCNB1.
Key Feature
Clonality
Polyclonal
Isotype
Ig
Host Species
Rabbit
Tested Applications
IHC-PWB
For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:50~100:
Western blot analysis in MDA-MB231 cell line lysates (35ug/lane).
Application
CCNB1 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human uterus tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.
Application
Western Blot at 1:1000 dilution + K562 whole cell lysate Lysates/proteins at 20 ug per lane.
Application Notes
For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:50~100:
Additional Information
Form
Liquid
Storage Instructions
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Storage Buffer
Supplied in PBS with 0.09% (W/V) sodium azide.
Note: The product is for research use only,not for use in diagnostic or therapeutic procedures.
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