Calreticulin Antibody

Product Profile

Product Name	Calreticulin Antibody
Antibody Type	Primary Antibodies
Product description	Calreticulin is involved in regulation of intracellular Ca2+ homoeostasis and ER Ca2+ capacity. It constitutes together with calnexin and ERp57 the ‘calreticulin/calnexin cycle’ that is responsible for folding and quality control of newly synthesized glycoproteins. Calreticulin has been implicated to play a role in many biological systems, including functions inside and outside the ER, indicating that the protein is a multi-process molecule.
Immunogen	Recombinant human calreticulin.

Key Feature

Clonality	Monoclonal
Isotype	IgG1, k
Host Species	Mouse
Tested Applications	ELISAWB
	ELISA: (direct and indirect: 1:2,000-1:5,000). Western Blot: (1:2,000-1:5,000 using ECL. Suggested blocking and dilution buffer is PBST containing 0.05% Tween 20 and 5% skim milk. Suggested incubation time is 1 hour at room temperature). Optimal conditions must be determined individually for each application.:
Species Reactivity	Human
Concentration	1 mg/ml
Purification

Target Information

Gene Symbol	CALR
Alternative Names	CRP55 Calregulin Endoplasmic Reticulum Resident Protein 60 ERp60 grp60
Tissue Specificity	Recognizes human calreticulin.

Database Links

Entrez Gene	811
Protein Accession	NP_004334

Application

Application Notes	ELISA: (direct and indirect: 1:2,000-1:5,000). Western Blot: (1:2,000-1:5,000 using ECL. Suggested blocking and dilution buffer is PBST containing 0.05% Tween 20 and 5% skim milk. Suggested incubation time is 1 hour at room temperature). Optimal conditions must be determined individually for each application.:

Additional Information

Form	Liquid
Storage Instructions	Stable for at least 1 year after receipt when stored at -20˚C.
Storage Buffer	Liquid. 0.2um-filtered solution in PBS, pH 7.4. Contains no preservatives.

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• Data sheet for OM273320