DDB1

Catalog Number：OM638961

  • Fig1: Western blot analysis of DDB1 on different cell lysate using anti-DDB1 antibody at 1/1,000 dilution. Positive control： Lane1: Mouse colon tissue Lane2: PC-12 Lane3: Siha

  • Fig2: ICC staining DDB1 in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

  • Fig3: ICC staining DDB1 in SH-SY5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

  • Fig4: ICC staining DDB1 in SK-Br-3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

  • Fig5: Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-DDB1 antibody. Counter stained with hematoxylin.

  • Fig6: Immunohistochemical analysis of paraffin-embedded human breast tissue using anti-DDB1 antibody. Counter stained with hematoxylin.

  • Fig7: Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-DDB1 antibody. Counter stained with hematoxylin.

  • Fig8: Flow cytometric analysis of K562 cells with DDB1 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black).

Product Profile

Product Name DDB1
Antibody Type Primary Antibodies
Product description 
Damaged DNA binding protein (DDB) is a heterodimer composed of two subunits, p127 and p48, which are designated DDB1 and DDB2, respectively. The DDB heterodimer is involved in repairing DNA damaged by ultraviolet light. Specifically, DDB, also designated UV-damaged DNA binding protein (UV-DDB), xeroderma pigmentosum group E binding factor (XPE-BF) and hepatitis B virus X-associated protein 1 (XAP-1), binds to damaged cyclobutane pyrimidine dimers (CPDs). Mutations in the DDB2 gene are implicated as causes of xeroderma pigmentosum group E, an autosomal recessive disease in which patients are defective in nucleotide excision DNA repair. XPE is characterized by hypersensitivity of the skin to sunlight with a high frequency of skin cancer as well as neurologic abnormalities. The hepatitis B virus (HBV) X protein interacts with DDB1, which may mediate HBx transactivation.
Immunogen 
Recombinant protein

Key Feature

Clonality Polyclonal
Isotype IgG
Host Species Rabbit
Tested Applications

WBICCIHCFC
WB:1:500-1:1000
ICC:1:100-1:500
IHC:1:100-1:500
FC:1:50-1:100
Species Reactivity

HumanMouseRat
Concentration 1 mg/mL.

Target Information

Alternative Names
Damage specific DNA binding protein 1 antibody Damage-specific DNA-binding protein 1 antibody DDB 1 antibody DDB p127 subunit antibody Ddb1 antibody DDB1_HUMAN antibody DDBa antibody DNA damage binding protein 1 antibody DNA damage-binding protein 1 antibody DNA damage-binding protein a antibody HBV X-associated protein 1 antibody UV damaged DNA binding factor antibody UV damaged DNA binding protein 1 antibody UV DDB 1 antibody UV DDB1 antibody UV-damaged DNA-binding factor antibody UV-damaged DNA-binding protein 1 antibody UV-DDB 1 antibody X associated protein 1 antibody XAP 1 antibody XAP-1 antibody XAP1 antibody Xeroderma pigmentosum group E complementing protein antibody Xeroderma pigmentosum group E-complementing protein antibody XPCe antibody XPE antibody XPE BF antibody XPE binding factor antibody XPE-BF antibody XPE-binding factor antibody
Molecular Weight(MW) 127 kDa, additional band 150kDa
Cellular Localization Nucleus. Cytoplasm.

Database Links

SwissProt ID Q16531
Q3U1J4
Q9ESW0

Application

  • Application

    Fig1: Western blot analysis of DDB1 on different cell lysate using anti-DDB1 antibody at 1/1,000 dilution. Positive control： Lane1: Mouse colon tissue Lane2: PC-12 Lane3: Siha

  • Application

    Fig2: ICC staining DDB1 in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

  • Application

    Fig3: ICC staining DDB1 in SH-SY5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

  • Application

    Fig4: ICC staining DDB1 in SK-Br-3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

  • Application

    Fig5: Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-DDB1 antibody. Counter stained with hematoxylin.

  • Application

    Fig6: Immunohistochemical analysis of paraffin-embedded human breast tissue using anti-DDB1 antibody. Counter stained with hematoxylin.

  • Application

    Fig7: Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-DDB1 antibody. Counter stained with hematoxylin.

  • Application

    Fig8: Flow cytometric analysis of K562 cells with DDB1 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black).

Positive Control Mouse colon tissue lysate, PC-12, Siha, A549, SH-SY5Y, SK-Br-3, K562, human breast, human kidney, rat brain.
Application Notes
WB:1:500-1:1000
ICC:1:100-1:500
IHC:1:100-1:500
FC:1:50-1:100

Additional Information

Form Liquid
Storage Instructions Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃ Avoid repeated freeze / thaw cycles.
Storage Buffer 1*TBS (pH7.4), 0.5%BSA, 50%Glycerol. Preservative: 0.05% Sodium Azide.
Note: The product is for research use only,not for use in diagnostic or therapeutic procedures.

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  • Catalog Number: OM638961
  • Price: $336.00
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  • Lead time: 4~6 Week

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