All products Antibodies E2A Antibody (V-18)

E2A Antibody (V-18)

Catalog Number：OM166034

  • Immunoperoxidase staining of formalin fixed, paraffin-embedded human lymph node tissue showing nuclear staining of reaction center cells at low (A) and high (B) magnification. Kindly provided by The Swedish Human Protein Atlas (HPA) program.

  • Western blot analysis of E2A expression in non-transfected 293T: (A), mouse E2A transfected 293T: (B) and Jurkat (C) whole cell lysates.

  • Western blot analysis of E2A expression in non-transfected 293T: (A), mouse E2A transfected 293T: (B) and Jurkat (C) whole cell lysates.

  • Western blot analysis of E2A expression in non-transfected: (A) and mouse E2A transfected: (B) 293 whole cell lysates.

  • Indirect, intracellular FCM analysis of fixed and permeabilized K-562 cells stained with E2A (V-18), followed by PE-conjugated goat anti-rabbit IgG: . Black line histogram represents the isotype control, normal rabbit IgG: .

  • Indirect, intracellular FCM analysis of fixed and permeabilized K-562 cells stained with E2A (V-18), followed by FITC-conjugated goat anti-rabbit IgG: . Black line histogram represents the isotype control, normal rabbit IgG: .

  • Antibodies tested include E2A (N-649): (A), E2A (Yae): (B) and E2A (V-18): (C,D).

  • Immunofluorescence staining of methanol-fixed K-562 cells showing nuclear staining.

  • Western blot analysis of E2A expression in Jurkat (A) and K-562 (B) whole cell lysates.

Product Profile

Product Name E2A Antibody (V-18)
Antibody Type Primary Antibodies
Modification Notes Differentiation of myogenic cells is regulated by multiple positively and negatively acting factors. One well characterized family of helix-loop-helix (HLH) proteins known to play an important role in the regulation of muscle cell development includes MyoD, myogenin, Myf-5 and Myf-6 (also designated MRF-4 or herculin). MyoD transcription factors form heterodimers with products of a more widely expressed family of bHLH genes, the E family, which consists of at least three distinct genes: E2A, IF2 and HEB. MyoD-E heterodimers bind avidly to consensus (CANNTG) E box target sites that are functionally important elements in the upstream regulatory sequences of many muscle-specific terminal differentiation genes. Both homo- and hetero-oligomers of these proteins are able to distinguish very closely related E box proteins and are believed to play important roles in lineage specific gene expression.

Key Feature

Clonality Polyclonal
Isotype IgG
Host Species Rabbit
Tested Applications

WBIPIFIHCFCELISAGSChIP
recommended for detection of E2A isoforms E12 and E47 of mouse, rat and human origin by WB, IP, IF, IHC(P), ELISA and indirect FCM using PE (sc-3739)- or FITC (sc-2012)-conjugated goat anti-rabbit IgG; also reactive with additional species, including equine, canine and bovine:
Species Reactivity

HumanMouseRat
Concentration 1mg/ml
Purification Affinity purified

Target Information

Alternative Names
epitope mapping at the C-terminus of E2A of human origin
Tissue Specificity epitope mapping at the C-terminus of E2A of human origin

Database Links

Entrez Gene 6929

Application

  • Application

    Immunoperoxidase staining of formalin fixed, paraffin-embedded human lymph node tissue showing nuclear staining of reaction center cells at low (A) and high (B) magnification. Kindly provided by The Swedish Human Protein Atlas (HPA) program.

  • Application

    Western blot analysis of E2A expression in non-transfected 293T: (A), mouse E2A transfected 293T: (B) and Jurkat (C) whole cell lysates.

  • Application

    Western blot analysis of E2A expression in non-transfected 293T: (A), mouse E2A transfected 293T: (B) and Jurkat (C) whole cell lysates.

  • Application

    Western blot analysis of E2A expression in non-transfected: (A) and mouse E2A transfected: (B) 293 whole cell lysates.

  • Application

    Indirect, intracellular FCM analysis of fixed and permeabilized K-562 cells stained with E2A (V-18), followed by PE-conjugated goat anti-rabbit IgG: . Black line histogram represents the isotype control, normal rabbit IgG: .

  • Application

    Indirect, intracellular FCM analysis of fixed and permeabilized K-562 cells stained with E2A (V-18), followed by FITC-conjugated goat anti-rabbit IgG: . Black line histogram represents the isotype control, normal rabbit IgG: .

  • Application

    Antibodies tested include E2A (N-649): (A), E2A (Yae): (B) and E2A (V-18): (C,D).

  • Application

    Immunofluorescence staining of methanol-fixed K-562 cells showing nuclear staining.

  • Application

    Western blot analysis of E2A expression in Jurkat (A) and K-562 (B) whole cell lysates.

Application Notes
recommended for detection of E2A isoforms E12 and E47 of mouse, rat and human origin by WB, IP, IF, IHC(P), ELISA and indirect FCM using PE (sc-3739)- or FITC (sc-2012)-conjugated goat anti-rabbit IgG; also reactive with additional species, including equine, canine and bovine:

Additional Information

Form Liquid
Storage Instructions For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Storage Buffer phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Note: The product is for research use only,not for use in diagnostic or therapeutic procedures.

Submit an review for OM166034
Submit an review for OM166034
  • Catalog Number: OM166034
  • Price: $336.00
  • Discount Price
  • Size:
  • Store: Out of stock
  • Lead time: 4~6 Week

  • Quantity:
    - +
  • Shipping to (Please select)
Add to cart
Add to Wishlist
Search the catalog number from any major antibody supplier/brand in the search bar above. The search engine will return OmnimAbs products for these targets.
PRODUCT CATEGORIES
Primary antibodies Secondary antibodies Tags antibodies Flow cytometry antibodies
SUPPORT
Catalogs Newsletter Order Online Buying FAQs Distributor
COMPANY
Profile Strategy Careers
CONTACT
Contact us

2013 © Omnimabs , All Rights Reserved.