Intracellular FCM analysis of fixed and permeabilized A-431 cells. Black line histogram represents the isotype control, normal rabbit IgG: .
Western blot analysis of ERK 3 ex
Immunofluorescence staining of methanol-fixed HeLa cells showing cytoplasmic localization.
Immunoperoxidase staining of formalin fixed, paraffin-em
Intracellular FCM analysis of fixed and permeabilized HeLa cells. Black line histogram represents the isotype control, normal rabbit IgG: .
Intracellular FCM analysis of fixed and permeabilized HeLa cells. Black line histogram represents the isotype control, normal rabbit IgG: .
Western blot analysis of ERK 3 ex
Immunoperoxidase staining of formalin-fixed, paraffin-em
Product Name | ERK 3 Antibody (I-15) PE |
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Antibody Type | Primary Antibodies |
Modification Notes | Mitogen-activated protein kinase (MAPK) signaling pathways involve closely related MAP kinases, including extracellular-signal-related kinase 3 (ERK 3, also designated PRKM6 and p97MAPK). Serum, growth factors and phorbol esters can initiate ERK 3 signaling pathways. Despite lacking a definitive nuclear localization sequence, ERK 3 constitutively localizes to the nucleus upon activation. p38 pathway activation-dependent upregulation of ERK 3 is independent of the status of p53, Bcl-2 and caspase-3 during cell stress and damage induced by proteasome inhibition, suggesting ERK 3 in part mediates intracellular defense or cell rescue. The human ERK 3 gene maps to chromosome 15q21.2 and encodes a 721 amino acid protein. |
Clonality | Polyclonal |
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Isotype | IgG |
Host Species | Rabbit |
Tested Applications | |
WB:1:100~1:500 IP:1:20~1:100 IF:1:20~1:100 IHC:1:10~1:50 |
|
Species Reactivity | |
Concentration | 1mg/ml |
Purification | Affinity purified |
Alternative Names | epitope mapping within an internal region of ERK 3 of rat origin |
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Tissue Specificity | epitope mapping within an internal region of ERK 3 of rat origin |
Entrez Gene | 5597 |
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Intracellular FCM analysis of fixed and permeabilized A-431 cells. Black line histogram represents the isotype control, normal rabbit IgG: .
Western blot analysis of ERK 3 ex
Immunofluorescence staining of methanol-fixed HeLa cells showing cytoplasmic localization.
Immunoperoxidase staining of formalin fixed, paraffin-em
Intracellular FCM analysis of fixed and permeabilized HeLa cells. Black line histogram represents the isotype control, normal rabbit IgG: .
Intracellular FCM analysis of fixed and permeabilized HeLa cells. Black line histogram represents the isotype control, normal rabbit IgG: .
Western blot analysis of ERK 3 ex
Immunoperoxidase staining of formalin-fixed, paraffin-em
Application Notes | WB:1:100~1:500 IP:1:20~1:100 IF:1:20~1:100 IHC:1:10~1:50 |
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Form | Liquid |
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Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
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