Flow cytometric analysis of A549 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Western Blot at 1:2000 dilution Lane 1: A549 whole cell lysate Lane 2: Hela whole cell lysate Lysates/proteins at 20 ug per lane.
Mutations in the winged-helix transcription factor gene at
the nude locus in mice and rats produce the pleiotropic phenotype
of hairlessness and athymia, resulting in a severely compromised
immune system. This gene is orthologous to the mouse and rat genes
and encodes a similar DNA-binding transcription factor that is
thought to regulate keratin gene expression. A mutation in this
gene has been correlated with T-cell immunodeficiency, the skin
disorder congenital alopecia, and nail dystrophy. Alternative
splicing in the 5' UTR of this gene has been observed. [provided by
RefSeq].1) Jugessur, A., et al. PLoS ONE(7), E11493 (2010) :
Immunogen
This FOXN1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 350-378 amino acids from the Central region of human FOXN1.
Key Feature
Clonality
Polyclonal
Isotype
Ig
Host Species
Rabbit
Tested Applications
FACSWB
For WB starting dilution is: 1:2000 For FACS starting dilution is: 1:10~50:
Flow cytometric analysis of A549 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Application
Western Blot at 1:2000 dilution Lane 1: A549 whole cell lysate Lane 2: Hela whole cell lysate Lysates/proteins at 20 ug per lane.
Application Notes
For WB starting dilution is: 1:2000 For FACS starting dilution is: 1:10~50:
Additional Information
Form
Liquid
Storage Instructions
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Storage Buffer
Supplied in PBS with 0.09% (W/V) sodium azide.
Note: The product is for research use only,not for use in diagnostic or therapeutic procedures.
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