Filamin-A

Catalog Number：OM638934

  • Fig1: Western blot analysis of Filamin-A on Hela (1) and NIH-3T3 (2) cell lysates using anti-Filamin-A antibody at 1/5,000 dilution.

  • Fig2: ICC staining Filamin-A in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

  • Fig3: ICC staining Filamin-A in HUVEC cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

  • Fig4: ICC staining Filamin-A in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

  • Fig5: Immunohistochemical analysis of paraffin-embedded human uterus tissue using anti-Filamin-A antibody. Counter stained with hematoxylin.

  • Fig6: Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue using anti-Filamin-A antibody. Counter stained with hematoxylin.

  • Fig7: Flow cytometric analysis of Hela cells with Filamin-A antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black).

Product Profile

Product Name Filamin-A
Antibody Type Primary Antibodies
Product description 
Caldesmon, Filamin 1, Nebulin and Villin are differentially expressed and regulated Actin binding proteins. Both muscular (CDh) and non-muscular (CDl) forms of Caldesmon have been identified and each has been shown to bind to Actin as well as to calmodulin and Myosin. CDh is expressed predominantly on thin filaments in smooth muscle, whereas CDl is widely expressed in non-muscle tissues and cells. Filamin 1, which is ubiquitously expressed and exists as a homodimer, functions to crosslink Actin to filaments. Nebulin is a large filamentous protein specific to muscle tissue that may function as a ruler for filament length. Several isoforms of Nebulin are produced by alternative exon usage. Villin is Ca2+-regulated and is the major structural component of the brush border of absorptive cells.
Immunogen 
Recombinant protein.

Key Feature

Clonality Polyclonal
Isotype IgG
Host Species Rabbit
Tested Applications

WBICCIHCFC
WB:1:500~1:2000
ICC:1:200~1:500
IHC:1:50~1:200
FC:1:200~1:500
Notes:Optimal dilutions/concentrations should be determined by the researcher.
Species Reactivity

HumanMouse
Concentration 1 mg/mL.

Target Information

Alternative Names
ABP 280 antibody ABP-280 antibody Actin-binding protein 280 antibody Alpha filamin antibody Alpha-filamin antibody APBX antibody CSBS antibody CVD1 antibody Endothelial actin binding protein antibody Endothelial actin-binding protein antibody Filamin 1 antibody Filamin A alpha antibody Filamin A antibody Filamin-1 antibody Filamin-A antibody FLN antibody FLN-A antibody FLN1 antibody FLNA antibody FLNA_HUMAN antibody FMD antibody MNS antibody NHBP antibody Non muscle filamin antibody Non-muscle filamin antibody OPD antibody OPD1 antibody OPD2 antibody XLVD antibody XMVD antibody
Molecular Weight(MW) 280 kDa
Cellular Localization Cytoplasm, cytoskeleton.

Database Links

SwissProt ID P21333
Q8BTM8

Application

  • Application

    Fig1: Western blot analysis of Filamin-A on Hela (1) and NIH-3T3 (2) cell lysates using anti-Filamin-A antibody at 1/5,000 dilution.

  • Application

    Fig2: ICC staining Filamin-A in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

  • Application

    Fig3: ICC staining Filamin-A in HUVEC cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

  • Application

    Fig4: ICC staining Filamin-A in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

  • Application

    Fig5: Immunohistochemical analysis of paraffin-embedded human uterus tissue using anti-Filamin-A antibody. Counter stained with hematoxylin.

  • Application

    Fig6: Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue using anti-Filamin-A antibody. Counter stained with hematoxylin.

  • Application

    Fig7: Flow cytometric analysis of Hela cells with Filamin-A antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black).

Positive Control Hela, NIH-3T3, HUVEC, MCF-7, human uterus tissue, human stomach cancer tissue.
Application Notes
WB:1:500~1:2000
ICC:1:200~1:500
IHC:1:50~1:200
FC:1:200~1:500
Notes:Optimal dilutions/concentrations should be determined by the researcher.

Additional Information

Form Liquid
Storage Instructions Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
Storage Buffer 1*TBS (pH7.4), 0.5%BSA, 50%Glycerol. Preservative: 0.05% Sodium Azide.
Note: The product is for research use only,not for use in diagnostic or therapeutic procedures.

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  • Catalog Number: OM638934
  • Price: $336.00
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  • Lead time: 4~6 Week

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