Formalin-fixed and paraffin-embedded human brain tissue reacted with GAS7 Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
Flow cytometric analysis of Jurkat cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Western blot analysis of GAS7 antibody in A2058 cell line lysates (35ug/lane)
Western blot analysis of GAS7 antibody in mouse cerebellum tissue lysates (35ug/lane)
Western Blot at 1:1000 dilution + human cerebellum lysate Lysates/proteins at 20 ug per lane.
Growth arrest-specific 7 is expressed primarily in terminally differentiated brain cells and predominantly in mature cerebellar Purkinje neurons. GAS7 plays a putative role in neuronal development.1) Chao,C.C., J. Neurosci. Res. 81 (2), 153-162 (2005)
Immunogen
This GAS7 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 438-465 amino acids from the C-terminal region of human GAS7.
Key Feature
Clonality
Polyclonal
Isotype
Ig
Host Species
Rabbit
Tested Applications
FACSIHC-PWB
For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:50~100 For FACS starting dilution is: 1:10~50:
Formalin-fixed and paraffin-embedded human brain tissue reacted with GAS7 Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
Application
Flow cytometric analysis of Jurkat cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Application
Western blot analysis of GAS7 antibody in A2058 cell line lysates (35ug/lane)
Application
Western blot analysis of GAS7 antibody in mouse cerebellum tissue lysates (35ug/lane)
Application
Western Blot at 1:1000 dilution + human cerebellum lysate Lysates/proteins at 20 ug per lane.
Application Notes
For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:50~100 For FACS starting dilution is: 1:10~50:
Additional Information
Form
Liquid
Storage Instructions
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Storage Buffer
Supplied in PBS with 0.09% (W/V) sodium azide.
Note: The product is for research use only,not for use in diagnostic or therapeutic procedures.
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