Western blot analysis of 20ng of HAT-tagged recombinant GGP1 protein using 1 μg of HAT antibody to immunoprecipitate and 1 μg/ml anti-GGP1 antibody to detect.
Western blot analysis of a HAT-tag-containing recombinant protein with HAT-tag antibody at (A) 0.125 and (B) 0.25 μg/ml.
Epitope tags provide a method to localize gene products in a variety of cell types, study the topology of proteins and protein complexes, identify associated proteins, and characterize newly identified, low abundance or poorly immunogenic proteins when protein specific antibodies are not available. HAT-tag is one of the more popular epitope tags for expressed recombinant proteins.
Immunogen
HAT-tag antibody was raised against a synthetic peptide containing KDHLIHNVHKEFHAHAHNK.
Key Feature
Clonality
Polyclonal
Isotype
IgG
Host Species
Rabbit
Tested Applications
ELISAIPWB
HAT-tag antibody can be used for detection of HAT-tag epitope-containing recombinant proteins by Western blot at 0.5 - 1 μ/ml.:
Western blot analysis of 20ng of HAT-tagged recombinant GGP1 protein using 1 μg of HAT antibody to immunoprecipitate and 1 μg/ml anti-GGP1 antibody to detect.
Application
Western blot analysis of a HAT-tag-containing recombinant protein with HAT-tag antibody at (A) 0.125 and (B) 0.25 μg/ml.
Positive Control
1) N/A
Application Notes
HAT-tag antibody can be used for detection of HAT-tag epitope-containing recombinant proteins by Western blot at 0.5 - 1 μ/ml.:
Additional Information
Form
Liquid
Storage Instructions
HAT-tag antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year.
Storage Buffer
HAT-tag antibody is supplied in PBS containing 0.02% sodium azide.
Note: The product is for research use only,not for use in diagnostic or therapeutic procedures.
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