Plasmid vectors for the expression of coding regions of eukaryotic genes in bacterial, insect and mammalian hosts are in common usage; such expression vectors are frequently used to encode hybrid fusion proteins consisting of a eukaryotic target protein and a specialized region designed to aid in the purification and visualization of the target protein. For example, the pCDM8 expression vector and derivatives thereof encode fusions between the target protein and an 11 amino acid peptide derived from the influenza protein hemagglutinin (HA). The HA epitope tag is useful in Western blotting and immunohistochemical localization of expressed fusion proteins when examined with antibodies raised specifically against the HA-epitope tag.
Key Feature
Clonality
Polyclonal
Isotype
IgG
Host Species
Rabbit
Tested Applications
WBIPIFFCELISAChIP
recommended for detection of proteins containing the HA tag by WB, IP, IF, FCM and ELISA:
Species Reactivity
HumanMouseRat
Concentration
1mg/ml
Purification
Affinity purified
Target Information
Alternative Names
epitope mapping within an internal region of the influenza hemagglutinin (HA) protein
Tissue Specificity
epitope mapping within an internal region of the influenza hemagglutinin (HA) protein
Application
Application
Western blot analysis of HA-probe expression in HA tagged β2B Tubulin (A), HA tagged 3pk (B) and HA tagged PTP 1B (C) transfected lysates.
Application
Antibodies tested include HA-probe (Y-11)-G: (A) and HA-probe (Y-11): (B,C).
Application
Immunofluorescence staining of methanol-fixed COS cells transfected with HA fusion protein showing cytoplasmic staining.
Application Notes
recommended for detection of proteins containing the HA tag by WB, IP, IF, FCM and ELISA:
Additional Information
Form
Liquid
Storage Instructions
For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
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