All products Antibodies HA tag antibody

HA tag antibody

Catalog Number：OM255791

  • Immunocytochemistry/Immunofluorescence: HA Epitope Tag Antibody - CHO cell

  • Immunoprecipitation: HA Epitope Tag Antibody - Immunoprecipitation: HA Epitope Tag Antibody- Samples: Lysate(150 mcg) from cells that were untransfected or stably transfected with a construct encoding an HA-tagged HIF 1-alpha truncation mutant. Affinity purified rabbit anti-HA used at 3mcg -150mcg lysate. Immunoprecipitated HA-HIF-1 alpha was detected using a monoclonal antibody to HIF-1 alpha.

  • Western Blot: HA Epitope Tag Antibody - 200, 100, or 50 ng of E. coli whole cell lysate expressing a multi-tag fusion protein. Antibody used at 0.04 ug/ml (1:25,000).

  • Western Blot: HA Epitope Tag Antibody - Western Blot: HA Epitope Tag Antibody-Samples: Extracts from S. Cerevisae co-transformed with plasmids expressing HA Tag G alpha and HA tagged RGS domain of RGS beta were screened by Western Blot to identify clones expressing both proteins. Antibody: affinity purifed rabbit anti-HA used at 0.1 mcg/ml

  • Western Blot: HA Epitope Tag Antibody - Detection of HA-tagged Protein by Western Blot. Samples: 200, 100, or 50 ng of E. coli whole cell lysate expressing a multitag fusion protein. Affinity purified rabbit anti-HA antibody used for WB at 0.04 ug/ml (1:25,000).

  • Western blot detection of 1 ug of recombinant E. coli protein (containing 11 different epitopes) using Lane 1 contains the antibody (1:1000) alone, lane 2 contains the antibody and neutralizing peptide.

Product Profile

Product Name HA tag antibody
Antibody Type Tags Antibodies
Product description 
Rabbit polyclonal to HA tagThe HA tag is derived from an epitope of the influenza hemagglutinin protein, which has been extensively used as a general epitope tag in expression vectors.
Immunogen 
YPYDVPDYA (influenza hemagglutinin-HA-epitope) conjugated to KLH

Key Feature

Clonality Polyclonal
Isotype IgG
Host Species Rabbit
Tested Applications

ELISAICC/IFIPWB
Suggested starting dilutions are as follows: WB: 1:1000-1:10000, ICC/IF: 1:100-1:1000, IP: 1:100-1:500, ELISA. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.:
Species Reactivity

Human
Concentration 1 mg/ml (Please refer to the vial label for the specific concentration)

Target Information

Gene Synonyms
HA epitope tag
HA tag
HA
Target HA tag
Tissue Specificity ELISA: The anti HA diluted 1:70,000 gave an O.D.=1.0 in a 15 minute reaction against peptide conjugated with a different carrier than used for anti peptide purification. HRP conjugated Goat anti rabbit IgG was used and TMB was the substrate.
Cellular Localization various

Application

  • Application

    Immunocytochemistry/Immunofluorescence: HA Epitope Tag Antibody - CHO cell

  • Application

    Immunoprecipitation: HA Epitope Tag Antibody - Immunoprecipitation: HA Epitope Tag Antibody- Samples: Lysate(150 mcg) from cells that were untransfected or stably transfected with a construct encoding an HA-tagged HIF 1-alpha truncation mutant. Affinity purified rabbit anti-HA used at 3mcg -150mcg lysate. Immunoprecipitated HA-HIF-1 alpha was detected using a monoclonal antibody to HIF-1 alpha.

  • Application

    Western Blot: HA Epitope Tag Antibody - 200, 100, or 50 ng of E. coli whole cell lysate expressing a multi-tag fusion protein. Antibody used at 0.04 ug/ml (1:25,000).

  • Application

    Western Blot: HA Epitope Tag Antibody - Western Blot: HA Epitope Tag Antibody-Samples: Extracts from S. Cerevisae co-transformed with plasmids expressing HA Tag G alpha and HA tagged RGS domain of RGS beta were screened by Western Blot to identify clones expressing both proteins. Antibody: affinity purifed rabbit anti-HA used at 0.1 mcg/ml

  • Application

    Western Blot: HA Epitope Tag Antibody - Detection of HA-tagged Protein by Western Blot. Samples: 200, 100, or 50 ng of E. coli whole cell lysate expressing a multitag fusion protein. Affinity purified rabbit anti-HA antibody used for WB at 0.04 ug/ml (1:25,000).

  • Application

    Western blot detection of 1 ug of recombinant E. coli protein (containing 11 different epitopes) using Lane 1 contains the antibody (1:1000) alone, lane 2 contains the antibody and neutralizing peptide.

Application Notes
Suggested starting dilutions are as follows: WB: 1:1000-1:10000, ICC/IF: 1:100-1:1000, IP: 1:100-1:500, ELISA. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.:

Additional Information

Form Liquid
Storage Instructions Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Storage Buffer 1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.
Note: The product is for research use only,not for use in diagnostic or therapeutic procedures.

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  • Catalog Number: OM255791
  • Price: $336.00
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  • Store: Out of stock
  • Lead time: 4~6 Week

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