All products Antibodies HA tag antibody

HA tag antibody

Catalog Number:OM255796

  • Immunofluorescence analysis of HA-recombinant protein transfected HeLa cell which fixed with paraformaldehyde, using HA tag antibody at 1:500 dilution.

  • HA tag antibody detects HA tag protein by western blot analysis. A. 30 µg 293T whole cell lysate/extract B. 30 µg whole cell lysate/extract of HA-human AKT-transfected 293T cells 7.5% SDS-PAGE HA tag antibody dilution: 1:5000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

  • An ELISA plate is coated with 50 μL of a DDDDK Tag conjugated BSA at concentration of 5 μg/mL. The peptide conjugated carrier protein is coated and detected with anti-DDDDK Tag antibody at concentration ranged from 3.1 to 200 ng/mL.

  • Immunoprecipitation of HA tag protein from 293T whole cell extracts using 5 μg of HA tag antibody . Western blot analysis was performed using HA tag antibody . EasyBlot anti-Rabbit IgG was used as a secondary reagent.

Product Profile

Product Name HA tag antibody
Antibody Type Tags Antibodies
Product description
Rabbit Polyclonal antibody to HA tag

Key Feature

Clonality Polyclonal
Isotype IgG
Host Species Rabbit
Tested Applications

ELISAICC/IFIPWBIB

*Optimal dilutions/concentrations should be determined by the researcher. :
Concentration 1 mg/ml (Please refer to the vial label for the specific concentration)
Purification Affinity purified

Target Information

Gene Synonyms
HA tag antibody
HA antibody
HA epitope tag antibody

Application

  • Application

    Immunofluorescence analysis of HA-recombinant protein transfected HeLa cell which fixed with paraformaldehyde, using HA tag antibody at 1:500 dilution.

  • Application

    HA tag antibody detects HA tag protein by western blot analysis. A. 30 µg 293T whole cell lysate/extract B. 30 µg whole cell lysate/extract of HA-human AKT-transfected 293T cells 7.5% SDS-PAGE HA tag antibody dilution: 1:5000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

  • Application

    An ELISA plate is coated with 50 μL of a DDDDK Tag conjugated BSA at concentration of 5 μg/mL. The peptide conjugated carrier protein is coated and detected with anti-DDDDK Tag antibody at concentration ranged from 3.1 to 200 ng/mL.

  • Application

    Immunoprecipitation of HA tag protein from 293T whole cell extracts using 5 μg of HA tag antibody . Western blot analysis was performed using HA tag antibody . EasyBlot anti-Rabbit IgG was used as a secondary reagent.

Positive Control HA-human AKT-transfected 293T
Application Notes
*Optimal dilutions/concentrations should be determined by the researcher. :

Additional Information

Form Liquid
Storage Instructions Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Storage Buffer 1XPBS, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
Note: The product is for research use only,not for use in diagnostic or therapeutic procedures.

  • Catalog Number: OM255796
  • Price: $336.00
  • Discount Price
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  • Store: Out of stock
  • Lead time: 4~6 Week
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