Immunofluorescence analysis of HA-recombinant protein transfected HeLa cell which fixed with paraformaldehyde, using HA tag antibody at 1:500 dilution.
HA tag antibody detects HA tag protein by western blot analysis. A. 30 µg 293T whole cell lysate/extract B. 30 µg whole cell lysate/extract of HA-human AKT-transfected 293T cells 7.5% SDS-PAGE HA tag antibody dilution: 1:5000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
An ELISA plate is coated with 50 μL of a DDDDK Tag conjugated BSA at concentration of 5 μg/mL. The peptide conjugated carrier protein is coated and detected with anti-DDDDK Tag antibody at concentration ranged from 3.1 to 200 ng/mL.
Immunoprecipitation of HA tag protein from 293T whole cell extracts using 5 μg of HA tag antibody . Western blot analysis was performed using HA tag antibody . EasyBlot anti-Rabbit IgG was used as a secondary reagent.
Product Name | HA tag antibody |
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Antibody Type | Tags Antibodies |
Product description |
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Clonality | Polyclonal |
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Isotype | IgG |
Host Species | Rabbit |
Tested Applications | |
*Optimal dilutions/concentrations should be determined by the researcher.
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Concentration | 1 mg/ml (Please refer to the vial label for the specific concentration) |
Purification | Affinity purified |
Gene Synonyms | HA tag antibody HA antibody HA epitope tag antibody |
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Immunofluorescence analysis of HA-recombinant protein transfected HeLa cell which fixed with paraformaldehyde, using HA tag antibody at 1:500 dilution.
HA tag antibody detects HA tag protein by western blot analysis. A. 30 µg 293T whole cell lysate/extract B. 30 µg whole cell lysate/extract of HA-human AKT-transfected 293T cells 7.5% SDS-PAGE HA tag antibody dilution: 1:5000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
An ELISA plate is coated with 50 μL of a DDDDK Tag conjugated BSA at concentration of 5 μg/mL. The peptide conjugated carrier protein is coated and detected with anti-DDDDK Tag antibody at concentration ranged from 3.1 to 200 ng/mL.
Immunoprecipitation of HA tag protein from 293T whole cell extracts using 5 μg of HA tag antibody . Western blot analysis was performed using HA tag antibody . EasyBlot anti-Rabbit IgG was used as a secondary reagent.
Positive Control | HA-human AKT-transfected 293T |
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Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
Form | Liquid |
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Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
Storage Buffer | 1XPBS, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
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