HA tag antibody [GT4810] immunoprecipitates HA tagged protein in IP experiments. IP Sample: 293T whole cell lysate/extract A : 20 μg whole cell lysate/extract of HA tagged protein expressing 293T cells B : Control with 2.5 μg of pre-immune mouse IgG C : Immunoprecipitation of HA tagged protein by 2.5 μg of HA tagg antibody 10% SDS-PAGE The immunoprecipitated HA tagged protein was detected by HA tag antibody [GT4810] diluted at 1 : 3000. EasyBlot anti-mouse IgG (HRP) was used as a secondary reagent.
HA tag antibody [GT4810] detects HA-recombinant protein by immunofluorescent analysis. Sample: HA tag transfected HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Red: HA-recombinant protein stained by HA tag antibody [GT4810] diluted at 1:500. Blue: Hoechst 33342 staining.
HA tag antibody [GT4810] detects HA tag protein by western blot analysis. A. 30 µg HeLa whole cell lysate/extract B. 30 µg whole cell lysate/extract of HA tagged protein expressing HeLa cells 5% SDS-PAGE HA tag antibody [GT4810] dilution: 1:5000 The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.
Product Name | HA tag antibody |
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Antibody Type | Tags Antibodies |
Product description |
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Clonality | Monoclonal |
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Isotype | IgG2a |
Clone Number | GT4810 |
Host Species | Mouse |
Tested Applications | |
*Optimal dilutions/concentrations should be determined by the researcher.
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Concentration | 1 mg/ml (Please refer to the vial label for the specific concentration) |
Purification | Affinity purified |
Gene Synonyms | HA tag antibody HA antibody HA epitope tag antibody |
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HA tag antibody [GT4810] immunoprecipitates HA tagged protein in IP experiments. IP Sample: 293T whole cell lysate/extract A : 20 μg whole cell lysate/extract of HA tagged protein expressing 293T cells B : Control with 2.5 μg of pre-immune mouse IgG C : Immunoprecipitation of HA tagged protein by 2.5 μg of HA tagg antibody 10% SDS-PAGE The immunoprecipitated HA tagged protein was detected by HA tag antibody [GT4810] diluted at 1 : 3000. EasyBlot anti-mouse IgG (HRP) was used as a secondary reagent.
HA tag antibody [GT4810] detects HA-recombinant protein by immunofluorescent analysis. Sample: HA tag transfected HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Red: HA-recombinant protein stained by HA tag antibody [GT4810] diluted at 1:500. Blue: Hoechst 33342 staining.
HA tag antibody [GT4810] detects HA tag protein by western blot analysis. A. 30 µg HeLa whole cell lysate/extract B. 30 µg whole cell lysate/extract of HA tagged protein expressing HeLa cells 5% SDS-PAGE HA tag antibody [GT4810] dilution: 1:5000 The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.
Positive Control | HA-tagged protein expressing HeLa cells |
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Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
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Form | Liquid |
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Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
Storage Buffer | PBS |
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