ICAD Antibody: Apoptosis is related to many diseases and induced by a family of cell death receptors and their ligands. Cell death signals are transduced by death domain containing adapter molecules and members of the caspase family of proteases. These death signals finally cause the degradation of chromosomal DNA by activated DNase. A human DNA fragmentation factor (DFF) was identified recently which was cleaved by caspase-3 during apoptosis. Mouse homologue of human DFF was identified as a DNase inhibitor designated ICAD, for inhibitor of caspase-activated DNase. Upon cleavage of DFF/ICAD, a caspase activated deoxyribonuclease (CAD) is released and activated and eventually causes the degradation of DNA in the nuclei. Therefore, the cleavage of CAD inhibitor molecule DFF/ICAD, which causes DNase activation and DNA degradation, is the hallmark of apoptotic cell death.1) Liu X, Zou H, Slaughter C, Wang X. DFF, a heterodimeric protein that functions downstream of caspase-3 to trigger DNA fragmentation during apoptosis. Cell 1997;89:175-184
Immunogen
ICAD antibody was raised against a 20 amino acid peptide near the carboxy terminus of mouse ICAD. The immunogen is located within the last 50 amino acids of ICAD.
Key Feature
Clonality
Polyclonal
Isotype
IgG
Host Species
Rabbit
Tested Applications
ELISAIFIHC-PWB
ICAD antibody can be used for detection of of ICAD by Western blot at 1:1000 dilution. A 45 kDa band can be detected. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 5 μg/mL.:
Immunohistochemistry of ICAD in mouse kidney tissue with ICAD antibody at 5 μg/ml.
Application
Immunofluorescence of ICAD in mouse kidney tissue with ICAD antibody at 5 μg/ml.
Application
Western blot analysis of ICAD in mouse lung (L), brain (B), liver (L), and kidney tissue lysate with CAD antibody at 1:1000 dilution.
Positive Control
1) Cat. No. 1402 - Mouse Lung Tissue Lysate
Application Notes
ICAD antibody can be used for detection of of ICAD by Western blot at 1:1000 dilution. A 45 kDa band can be detected. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 5 μg/mL.:
Additional Information
Form
Liquid
Storage Instructions
ICAD antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Storage Buffer
ICAD Antibody is supplied in PBS containing 0.02% sodium azide.
Note: The product is for research use only,not for use in diagnostic or therapeutic procedures.
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