IREB2

Catalog Number：OM638951

  • Fig1: Western blot analysis of IREB2 on different lysates using anti-IREB2 antibody at 1/500 dilution. Positive control： Lane1: K562 Lane2: Mouse kidney tissue Lane3: Rat skin tissue

  • Fig2: ICC staining IREB2 in 293T cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

  • Fig3: ICC staining IREB2 in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

  • Fig4: ICC staining IREB2 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

  • Fig5: Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-IREB2 antibody. Counter stained with hematoxylin.

  • Fig6: Immunohistochemical analysis of paraffin-embedded human placenta tissue using anti-IREB2 antibody. Counter stained with hematoxylin.

  • Fig7: Flow cytometric analysis of K562 cells with IREB2 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black).

Product Profile

Product Name IREB2
Antibody Type Primary Antibodies
Product description 
Iron metabolism is essential for sustaining mammalian homeostasis. Iron uptake and distribution is a highly regulated process in mammalian cells that is monitored by two iron sensing proteins: IRP-1 and -2 (iron regulatory protein-1 and -2), also known as iron responsive element-binding protein-1 and -2 (IRE-BP-1 and -2) or aconitase 1 and 2. IRP-1 and IRP-2 are important soluble regulatory factors that mediate iron uptake and storage in mammalian cells. They are capable of either repressing translation or enhancing mRNA stability by associating with stem-loop motifs known as iron-responsive elements (IREs). IRPs respond to stress mediators, iron concentration and signaling factors, including nitrogen monoxide, cytokines and hydrogen peroxide.
Immunogen 
Recombinant protein

Key Feature

Clonality Polyclonal
Isotype IgG
Host Species Rabbit
Tested Applications

WBICCIHCFC
WB:1:500-1:1000
ICC:1:50-1:200
IHC:1:50-1:200
FC:1:50-1:100
Species Reactivity

HumanMouseRat
Concentration 1 mg/mL.

Target Information

Alternative Names
ACO3 antibody FLJ23381 antibody IRE BP2 antibody IRE-BP 2 antibody IREB 2 antibody IREB2 antibody IREB2_HUMAN antibody Iron regulatory protein 2 antibody iron responsive element binding protein 2 antibody Iron-responsive element-binding protein 2 antibody IRP 2 antibody IRP2 antibody IRP2AD antibody OTTHUMP00000185022 antibody
Molecular Weight(MW) 105/37 kDa
Cellular Localization Cytoplasm.

Database Links

SwissProt ID P48200
Q811J3
Q62751

Application

  • Application

    Fig1: Western blot analysis of IREB2 on different lysates using anti-IREB2 antibody at 1/500 dilution. Positive control： Lane1: K562 Lane2: Mouse kidney tissue Lane3: Rat skin tissue

  • Application

    Fig2: ICC staining IREB2 in 293T cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

  • Application

    Fig3: ICC staining IREB2 in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

  • Application

    Fig4: ICC staining IREB2 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

  • Application

    Fig5: Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-IREB2 antibody. Counter stained with hematoxylin.

  • Application

    Fig6: Immunohistochemical analysis of paraffin-embedded human placenta tissue using anti-IREB2 antibody. Counter stained with hematoxylin.

  • Application

    Fig7: Flow cytometric analysis of K562 cells with IREB2 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black).

Positive Control K562, mouse kidney tissue lysate, rat skin tissue lysate, 293T, A549, HepG2, human kidney tissue, human placenta tissue.
Application Notes
WB:1:500-1:1000
ICC:1:50-1:200
IHC:1:50-1:200
FC:1:50-1:100

Additional Information

Form Liquid
Storage Instructions Store at +4℃after thawing. Aliquot store at -20℃or -80℃. Avoid repeated freeze / thaw cycles.
Storage Buffer 1*TBS (pH7.4), 0.5%BSA, 50%Glycerol. Preservative: 0.05% Sodium Azide.
Note: The product is for research use only,not for use in diagnostic or therapeutic procedures.

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  • Catalog Number: OM638951
  • Price: $336.00
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  • Lead time: 4~6 Week

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