The activation of Toll-like receptors (TLRs) is central to innate and adaptive immunity. All TLRs use the adaptor MyD88 for signaling. IRF-5 is generally involved downstream of the TLR-MyD88 signalling pathway for gene induction of proinflammatory cytokines, such as interleukin-6 (IL-6), IL-12 and tumour-necrosis factor-alpha.1) Takaoka, A., et al., (2005) Nature 434 (7030), 243-249.
Immunogen
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of mouse IRF5.
Key Feature
Clonality
Polyclonal
Host Species
Rabbit
Tested Applications
ELISAWB
IRF5 antibody can be used for detection of IRF5 by ELISA at 1:62500. IRF5 antibody can be used for detection of IRF5 by western blot at 0.15 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.:
Antibody used in WB on Human Lymphnode at 0.15 ug/ml.
Positive Control
1) Cat. No. 1369 - Human Lymph node Tissue Lysate
Application Notes
IRF5 antibody can be used for detection of IRF5 by ELISA at 1:62500. IRF5 antibody can be used for detection of IRF5 by western blot at 0.15 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.:
Additional Information
Form
Liquid
Storage Instructions
For short periods of storage (days) store at 4˚C. For longer periods of storage, store IRF5 antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles.
Storage Buffer
Antibody is lyophilized in PBS buffer with 2% sucrose. Add 50 μL of distilled water. Final antibody concentration is 1 mg/mL.
Note: The product is for research use only,not for use in diagnostic or therapeutic procedures.
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