MLH1 is a DNA mismatch repair protein that heterodimerizes with PMS2 to form MutL alpha, a component of the post-replicative DNA mismatch repair system (MMR). DNA repair is initiated by MutS alpha (MSH2-MSH6) or MutS beta (MSH2-MSH6) binding to a dsDNA mismatch, and then MutL alpha is recruited to the heteroduplex. Assembly of the MutL-MutS-heteroduplex ternary complex in the presence of RFC and PCNA is sufficient to activate endonuclease activity of PMS2. It introduces single-strand breaks near the mismatch and thus generates new entry points for the exonuclease EXO1 to degrade the mismatch strand. MutL alpha (MLH1-PMS2) interacts physically with the clamp loader subunits of DNA polymerase III, suggesting that it may recruit DNA polymerase III to the site of the MMR. Defects in MLH1 are the cause of hereditary nonpolyposis colorectal cancer type 2 (HNPCC2). Most patients with HNPCC have mutations in either the MLH1 or MSH2 genes (1).
Immunogen
peptide
Key Feature
Clonality
Polyclonal
Isotype
IgG
Host Species
Rabbit
Tested Applications
WBICCIHCFCIP
WB:1:1,000 IP:1:10-100 FC:1:10-100
Species Reactivity
HumanMouseRat
Concentration
1 mg/mL.
Target Information
Alternative Names
COCA 2 antibody
COCA2 antibody
DNA mismatch repair protein Mlh1 antibody
FCC 2 antibody
FCC2 antibody
hMLH 1 antibody
hMLH1 antibody
HNPCC 2 antibody
HNPCC antibody
HNPCC2 antibody
MGC5172 antibody
MLH 1 antibody
MLH1 antibody
MLH1_HUMAN antibody
MutL homolog 1 (E. coli) antibody
MutL homolog 1 antibody
MutL homolog 1 colon cancer nonpolyposis type 2 antibody
MutL homolog 1 colon cancer nonpolyposis type 2 (E. coli) antibody
MutL protein homolog 1 antibody
MutL E. coli homolog of 1 antibody
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