All products Antibodies MRP8/S100A8

MRP8/S100A8

Catalog Number：OM638960

  • Fig1: Western blot analysis of MRP8/S100A8 on HL-60 cell lysate using anti-MRP8/S100A8 antibody at 1/100 dilution.

  • Fig2: ICC staining MRP8/S100A8 in AGS cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

  • Fig3: ICC staining MRP8/S100A8 in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

  • Fig4: ICC staining MRP8/S100A8 in SK-Br-3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

  • Fig5: Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-MRP8/S100A8 antibody. Counter stained with hematoxylin.

  • Fig6: Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-MRP8/S100A8 antibody. Counter stained with hematoxylin.

  • Fig7: Immunohistochemical analysis of paraffin-embedded rat spleen tissue using anti-MRP8/S100A8 antibody. Counter stained with hematoxylin.

  • Fig8: Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-MRP8/S100A8 antibody. Counter stained with hematoxylin.

  • Fig9: Immunohistochemical analysis of paraffin-embedded human breast cancer tissue using anti-MRP8/S100A8 antibody. Counter stained with hematoxylin.

  • Fig10: Flow cytometric analysis of THP-1 cells with MRP8/S100A8 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black).

Product Profile

Product Name MRP8/S100A8
Antibody Type Primary Antibodies
Product description 
The family of EF-hand type Ca2+-binding proteins includes Calbindin (previously designated vitamin D-dependent Ca2+-binding protein), S-100 α and β, Cal-granulins A (also designated MRP8), B (also designated MRP14) and C (S-100 like proteins) and the parvalbumin family members, including parvalbumin α and parvalbumin β (also designated oncomodulin). Calbindin, S-100 proteins and parvalbumin proteins are each expressed in neural tissues. In addition, S-100 α and β are present in a variety of other tissues and Calbindin is present in intestine and kidney. Parvalbumin α is also found in fast-contracting/ relaxing skeletal muscle fibers and parvalbumin β is found in many tumor tissues as well as in the organ of Corti. Calbindin, S-100 proteins and parvalbulmins have all been detected in Leydig cells and the testis. These proteins are thought to play a role in hormone production and spermatogenesis. Calgranulin is expressed in macrophages and epithelial cells.
Immunogen 
Recombinant protein

Key Feature

Clonality Polyclonal
Isotype IgG
Host Species Rabbit
Tested Applications

WBICCIHCFC
WB:1:500
ICC:1:50-1:200
IHC:1:50-1:200
FC:1:50-1:100
Species Reactivity

HumanMouseRat
Concentration 1 mg/mL.

Target Information

Alternative Names
60B8Ag antibody AI323541 antibody B8Ag antibody BEE11 antibody CAGA antibody Calgranulin-A antibody Calprotectin L1L subunit antibody Calprotectin
included antibody CFAG antibody CGLA antibody Chemotactic cytokine CP-10 antibody CP-10 antibody Cystic fibrosis antigen antibody L1Ag antibody Leukocyte L1 complex light chain antibody MA387 antibody MIF antibody Migration inhibitory factor-related protein 8 antibody MRP-8 antibody Myeloid-related protein 8 antibody Neutrophil cytosolic 7 kDa protein antibody NIF antibody p8 antibody Pro-inflammatory S100 cytokine antibody Protein S100-A8 antibody S100 calcium binding protein A8 (calgranulin A) antibody S100 calcium binding protein A8 antibody S100 calcium-binding protein A8 antibody S100A8 antibody S100A8/S100A9 complex
included antibody S10A8_HUMAN antibody Urinary stone protein band A antibody
Molecular Weight(MW) 11 kDa
Cellular Localization Cell membrane. Cytoplasm. Secreted.

Database Links

SwissProt ID P05109
P27005

Application

  • Application

    Fig1: Western blot analysis of MRP8/S100A8 on HL-60 cell lysate using anti-MRP8/S100A8 antibody at 1/100 dilution.

  • Application

    Fig2: ICC staining MRP8/S100A8 in AGS cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

  • Application

    Fig3: ICC staining MRP8/S100A8 in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

  • Application

    Fig4: ICC staining MRP8/S100A8 in SK-Br-3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

  • Application

    Fig5: Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-MRP8/S100A8 antibody. Counter stained with hematoxylin.

  • Application

    Fig6: Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-MRP8/S100A8 antibody. Counter stained with hematoxylin.

  • Application

    Fig7: Immunohistochemical analysis of paraffin-embedded rat spleen tissue using anti-MRP8/S100A8 antibody. Counter stained with hematoxylin.

  • Application

    Fig8: Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-MRP8/S100A8 antibody. Counter stained with hematoxylin.

  • Application

    Fig9: Immunohistochemical analysis of paraffin-embedded human breast cancer tissue using anti-MRP8/S100A8 antibody. Counter stained with hematoxylin.

  • Application

    Fig10: Flow cytometric analysis of THP-1 cells with MRP8/S100A8 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black).

Positive Control HL-60, AGS, MCF-7, SK-Br-3, human tonsil tissue, human colon cancer tissue, human spleen tissue, human breast cancer tissue, rat spleen tissue, THP-1.
Application Notes
WB:1:500
ICC:1:50-1:200
IHC:1:50-1:200
FC:1:50-1:100

Additional Information

Form Liquid
Storage Instructions Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
Storage Buffer 1*TBS (pH7.4), 0.5%BSA, 50%Glycerol. Preservative: 0.05% Sodium Azide.
Note: The product is for research use only,not for use in diagnostic or therapeutic procedures.

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  • Catalog Number: OM638960
  • Price: $336.00
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  • Lead time: 4~6 Week

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