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Myc tag antibody

Catalog Number：OM264286

  • Myc antibody Immunoprecipitation of Myc tagged protein. HeLa transfected lysates were subjected to immunoprecipitation using (B) normal rabbit IgG or (C) 2.5 ug of anti-Myc antibody . (A) Input, 15ug of HeLa transfected lysates. The precipitated protein was detected by diluted at 1:10000. EasyBlot anti-Rabbit IgG Kit was used in Western blot.

  • Immunofluorescence analysis of paraformaldehyde-fixed Myc tag transfected HeLa, using Myc tag antibody (green) at 1:3000 dilution. Alpha-tubulin filaments were labeled with (red) at 1:2500.

  • Western blot analysis of extracts from untransfected control cells and transfected cells overexpressing Myc tagged protein, using Myc tag antibody. A: Non-transfected 293T lysate (10 µg) B: Transfected 293T lysate (10 µg) (~60 kDa) The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Product Profile

Product Name Myc tag antibody
Antibody Type Tags Antibodies
Product description 
Rabbit Polyclonal antibody to Myc tag
Immunogen 
Synthetic peptide corresponding to a region within amino acids 410 and 419 of human c-Myc

Key Feature

Clonality Polyclonal
Isotype IgG
Host Species Rabbit
Tested Applications

ICC/IFIPWB
*Optimal dilutions/concentrations should be determined by the researcher. :
Concentration 1 mg/ml (Please refer to the vial label for the specific concentration)
Purification Affinity purified

Target Information

Gene Synonyms
Myc tag antibody

Application

  • Application

    Myc antibody Immunoprecipitation of Myc tagged protein. HeLa transfected lysates were subjected to immunoprecipitation using (B) normal rabbit IgG or (C) 2.5 ug of anti-Myc antibody . (A) Input, 15ug of HeLa transfected lysates. The precipitated protein was detected by diluted at 1:10000. EasyBlot anti-Rabbit IgG Kit was used in Western blot.

  • Application

    Immunofluorescence analysis of paraformaldehyde-fixed Myc tag transfected HeLa, using Myc tag antibody (green) at 1:3000 dilution. Alpha-tubulin filaments were labeled with (red) at 1:2500.

  • Application

    Western blot analysis of extracts from untransfected control cells and transfected cells overexpressing Myc tagged protein, using Myc tag antibody. A: Non-transfected 293T lysate (10 µg) B: Transfected 293T lysate (10 µg) (~60 kDa) The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Positive Control 293T transfected myc-tag recombinant protein
Application Notes
*Optimal dilutions/concentrations should be determined by the researcher. :

Additional Information

Form Liquid
Storage Instructions Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Storage Buffer 1XPBS, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
Note: The product is for research use only,not for use in diagnostic or therapeutic procedures.

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  • Catalog Number: OM264286
  • Price: $336.00
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  • Lead time: 4~6 Week

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