Fig1: Western blot analysis of Myosin Light Chain 2 on different lysates using anti-Myosin Light Chain 2 antibody at 1/500 dilution. Positive control: Lane1: Mouse skeletal muscle Lane2: Human skeletal muscle Lane3: Mouse heart Lane4: Rat heart
Fig2: ICC staining Myosin Light Chain 2 in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Fig3: ICC staining Myosin Light Chain 2 in C2C12 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Fig4: Immunohistochemical analysis of paraffin-embedded mouse heart tissue using anti-Myosin Light Chain 2 antibody. Counter stained with hematoxylin.
Fig5: Immunohistochemical analysis of paraffin-embedded rat heart tissue using anti-Myosin Light Chain 2 antibody. Counter stained with hematoxylin.
Fig6: Flow cytometric analysis of Hela cells with Myosin Light Chain 2 antibody at 1/100 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Alexa Fluor 488-conjugated Goat anti rabbit IgG was used as the secondary antibody.
Product Name | Myosin Light Chain 2 |
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Antibody Type | Primary Antibodies |
Product description |
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Immunogen | Peptide |
Clonality | Polyclonal |
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Isotype | IgG |
Host Species | Rabbit |
Tested Applications | |
WB:1:1,000-1:2,000 ICC:1:50-1:200 IHC:1:50-1:200 FC:1:50-1:100 |
|
Species Reactivity | |
Concentration | 1 mg/mL. |
Alternative Names | Cardiac myosin light chain-2 antibody
Cardiac ventricular myosin light chain 2 antibody
CMH10 antibody
MLC 2v antibody
MLC-2 antibody
MLC-2v antibody
MLC2 antibody
MLRV_HUMAN antibody
MYL 2 antibody
MYL2 antibody
Myosin light chain 2 regulatory cardiac slow antibody
Myosin light polypeptide 2 regulatory cardiac slow antibody
Myosin regulatory light chain 2 antibody
Myosin regulatory light chain 2 ventricular/cardiac muscle isoform antibody
Regulatory light chain of myosin antibody
RLC of myosin antibody
Slow cardiac myosin regulatory light chain 2 antibody
ventricular/cardiac muscle isoform antibody
|
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Molecular Weight(MW) | 19 kDa |
Cellular Localization | Cytoplasm. |
SwissProt ID | P10916 P51667 P08733 |
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Fig1: Western blot analysis of Myosin Light Chain 2 on different lysates using anti-Myosin Light Chain 2 antibody at 1/500 dilution. Positive control: Lane1: Mouse skeletal muscle Lane2: Human skeletal muscle Lane3: Mouse heart Lane4: Rat heart
Fig2: ICC staining Myosin Light Chain 2 in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Fig3: ICC staining Myosin Light Chain 2 in C2C12 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Fig4: Immunohistochemical analysis of paraffin-embedded mouse heart tissue using anti-Myosin Light Chain 2 antibody. Counter stained with hematoxylin.
Fig5: Immunohistochemical analysis of paraffin-embedded rat heart tissue using anti-Myosin Light Chain 2 antibody. Counter stained with hematoxylin.
Fig6: Flow cytometric analysis of Hela cells with Myosin Light Chain 2 antibody at 1/100 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Alexa Fluor 488-conjugated Goat anti rabbit IgG was used as the secondary antibody.
Positive Control | Mouse skeletal muscle tissue lysate, human skeletal muscle tissue lysate, mouse heart tissue, rat heart tissue, A549, Hela, C2C12. |
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Application Notes | WB:1:1,000-1:2,000 ICC:1:50-1:200 IHC:1:50-1:200 FC:1:50-1:100 |
Form | Liquid |
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Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
Storage Buffer | 1*TBS (pH7.4), 0.5%BSA, 50%Glycerol. Preservative: 0.05% Sodium Azide. |
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