Western blot analysis of NCF1C Antibody (C-term) in T47D cell line lysates (35ug/lane). NCF1C (arrow) was detected using the purified Pab.
Formalin-fixed and paraffin-embedded human breast carcinoma reacted with NCF1C Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
NCF1C Antibody (C-term) flow cytometry analysis of MDA-MB231 cells (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Product Name | NCF1C Antibody (C-term) |
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Antibody Type | Primary Antibodies |
Clonality | Polyclonal |
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Isotype | Ig |
Host Species | Rabbit |
Tested Applications | |
WB~~1:100~500 IHC~~1:50~100 FC~~1:10~50: |
|
Species Reactivity | |
Concentration | 1 mg/ml |
Purification |
Gene Synonyms | SH3PXD1C |
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Alternative Names | NCF1C SH3PXD1C Putative neutrophil cytosol factor 1C Putative SH3 and PX domain-containing protein 1C |
Molecular Weight(MW) | 41851 Da |
Function | May be required for activation of the latent NADPH oxidase (necessary for superoxide production) (By similarity) |
Tissue Specificity | This NCF1C antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 283~312 amino acids from the C-terminal region of human NCF1C. |
Cellular Localization | Cytoplasm (By similarity). |
Western blot analysis of NCF1C Antibody (C-term) in T47D cell line lysates (35ug/lane). NCF1C (arrow) was detected using the purified Pab.
Formalin-fixed and paraffin-embedded human breast carcinoma reacted with NCF1C Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
NCF1C Antibody (C-term) flow cytometry analysis of MDA-MB231 cells (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Application Notes | WB~~1:100~500 IHC~~1:50~100 FC~~1:10~50: |
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Form | Liquid |
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Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
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