All products Antibodies NDEL1 Antibody (Center)

NDEL1 Antibody (Center)

Catalog Number：OM215082

  • Western blot analysis of NDEL1 Antibody (Center) in A375 cell line lysates (35ug/lane). NDEL1 (arrow) was detected using the purified Pab.

  • Formalin-fixed and paraffin-embedded human brain reacted with NDEL1 Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

  • Western blot analysis of NDEL1 (arrow) using rabbit polyclonal NDEL1 Antibody (Center) . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the NDEL1 gene.

  • NDEL1 Antibody (Center) flow cytometric analysis of 293 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Product Profile

Product Name NDEL1 Antibody (Center)
Antibody Type Primary Antibodies

Key Feature

Clonality Polyclonal
Isotype Ig
Host Species Rabbit
Tested Applications

WBIHCFC
WB~~1:100~500 IHC~~1:50~100 FC~~1:10~50:
Species Reactivity

Human
Concentration 1 mg/ml
Purification

Target Information

Gene Synonyms
EOPA
MITAP1
NUDEL
Alternative Names
NDEL1
EOPA
MITAP1
NUDEL
Nuclear distribution protein nudE-like 1
Mitosin-associated protein 1
Molecular Weight(MW) 38375 Da
Function
Required for organization of the cellular microtubule array and microtubule anchoring at the centrosome. May regulate microtubule organization at least in part by targeting the microtubule severing protein KATNA1 to the centrosome. Also positively regulates the activity of the minus-end directed microtubule motor protein dynein. May enhance dynein-mediated microtubule sliding by targeting dynein to the microtubule plus ends. Required for several dynein- and microtubule-dependent processes such as the maintenance of Golgi integrity, the centripetal motion of secretory vesicles and the coupling of the nucleus and centrosome. Also required during brain development for the migration of newly formed neurons from the ventricular/subventricular zone toward the cortical plate. Plays a role, together with DISC1, in the regulation of neurite outgrowth Required for mitosis in some cell types but appears to be dispensible for mitosis in cortical neuronal progenitors, which instead requires NDE1. Facilitates the polymerization of neurofilaments from the individual subunits NEFH and NEFL
Tissue Specificity Expressed in brain, heart, kidney, liver, lung, pancreas, placenta and skeletal muscle
Cellular Localization Cytoplasm, cytoskeleton. Cytoplasm, cytoskeleton, centrosome. Chromosome, centromere, kinetochore Cytoplasm, cytoskeleton, spindle. Note=Localizes to the cell body of the motor neurons and colocalizes with assembled neurofilaments within axonal processes. Localizes to the microtubules of the manchette in elongated spermatids. Colocalizes with DISC1 in the perinuclear region, including the centrosome (By similarity) Localizes to the interphase centrosome and the mitotic spindle Localizes to the kinetochore in a CENPF-dependent manner

Database Links

Entrez Gene 81565

Application

  • Application

    Western blot analysis of NDEL1 Antibody (Center) in A375 cell line lysates (35ug/lane). NDEL1 (arrow) was detected using the purified Pab.

  • Application

    Formalin-fixed and paraffin-embedded human brain reacted with NDEL1 Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

  • Application

    Western blot analysis of NDEL1 (arrow) using rabbit polyclonal NDEL1 Antibody (Center) . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the NDEL1 gene.

  • Application

    NDEL1 Antibody (Center) flow cytometric analysis of 293 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Application Notes
WB~~1:100~500 IHC~~1:50~100 FC~~1:10~50:

Additional Information

Form Liquid
Storage Instructions For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Storage Buffer Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Note: The product is for research use only,not for use in diagnostic or therapeutic procedures.

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  • Catalog Number: OM215082
  • Price: $336.00
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  • Lead time: 2~3 Week

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