All products Antibodies NFIA Antibody

NFIA Antibody

Catalog Number：OM286099

  • Fluorescent confocal image of Hela cell stained with NFIA Antibody .Hela cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with NFIA primary antibody (1:25). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin

  • Western blot analysis in HepG2 cell line lysates (35ug/lane).

Product Profile

Product Name NFIA Antibody
Antibody Type Primary Antibodies
Product description 
Nuclear factor I (NFI) proteins constitute a family of
dimeric DNA-binding proteins with similar, and possibly identical,
DNA-binding specificity. They function as cellular transcription
factors and as replication factors for adenovirus DNA replication.
Diversity in this protein family is generated by multiple genes,
differential splicing, and heterodimerization.1) Bailey, S.D., et al. Diabetes Care 33(10):2250-2253(2010)
Immunogen 
This NFIA antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 470-498 amino acids from the C-terminal region of human NFIA.

Key Feature

Clonality Polyclonal
Isotype Ig
Host Species Rabbit
Tested Applications

IFWB
For WB starting dilution is: 1:1000 For IF starting dilution is: 1:10~50:
Species Reactivity

Human
Concentration 1 mg/ml
Purification Affinity purified

Target Information

Gene Symbol NFIA
Alternative Names
Nuclear factor 1 A-type
NF1-A
Nuclear factor 1/A
CCAAT-box-binding transcription factor
CTF
Nuclear factor I/A
NF-I/A
NFI-A
TGGCA-binding protein
NFIA
KIAA1439
Molecular Weight(MW) 56 kDa
Sequence Similarities Predicted species reactivity based on immunogen sequence: Chicken

Database Links

Entrez Gene 4774
Protein Accession Q12857

Application

  • Application

    Fluorescent confocal image of Hela cell stained with NFIA Antibody .Hela cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with NFIA primary antibody (1:25). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin

  • Application

    Western blot analysis in HepG2 cell line lysates (35ug/lane).

Application Notes
For WB starting dilution is: 1:1000 For IF starting dilution is: 1:10~50:

Additional Information

Form Liquid
Storage Instructions Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Storage Buffer Supplied in PBS with 0.09% (W/V) sodium azide.
Note: The product is for research use only,not for use in diagnostic or therapeutic procedures.

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  • Catalog Number: OM286099
  • Price: $336.00
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