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NGLY1 Antibody (N-term) western blot analysis in K562 cell line lysates (35ug/lane).This demonstrates the NGLY1 antibody detected the NGLY1 protein (arrow).
NGLY1 Antibody (N-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the NGLY1 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.
NGLY1 Antibody (N-term) flow cytometric analysis of K562 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Product Profile
| Product Name | NGLY1 Antibody (N-term) |
|---|---|
| Antibody Type | Primary Antibodies |
Key Feature
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | |
WB~~1:100~500 IHC~~1:50~100 FC~~1:10~50: |
|
| Species Reactivity | |
| Concentration | 1 mg/ml |
| Purification |
Target Information
| Gene Synonyms | PNG1 |
|---|---|
| Alternative Names | NGLY1 PNG1 Peptide-N(4)-(N-acetyl-beta-glucosaminyl)asparagine amidase N-glycanase 1 Peptide:N-glycanase |
| Molecular Weight(MW) | 74390 Da |
| Function | Specifically deglycosylates the denatured form of N- linked glycoproteins in the cytoplasm and assists their proteasome-mediated degradation. Cleaves the beta-aspartyl- glucosamine (GlcNAc) of the glycan and the amide side chain of Asn, converting Asn to Asp. Prefers proteins containing high- mannose over those bearing complex type oligosaccharides. Can recognize misfolded proteins in the endoplasmic reticulum that are exported to the cytosol to be destroyed and deglycosylate them, while it has no activity toward native proteins. Deglycosylation is a prerequisite for subsequent proteasome-mediated degradation of some, but not all, misfolded glycoproteins |
| Tissue Specificity | This NGLY1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 92-122 amino acids from the N-terminal region of human NGLY1. |
| Cellular Localization | Cytoplasm. |
Database Links
| Entrez Gene | 55768 |
|---|---|
| Nucleotide Accession | NP_060767 |
Application
-
Application
NGLY1 Antibody (N-term) western blot analysis in K562 cell line lysates (35ug/lane).This demonstrates the NGLY1 antibody detected the NGLY1 protein (arrow).
-
Application
NGLY1 Antibody (N-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the NGLY1 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.
-
Application
NGLY1 Antibody (N-term) flow cytometric analysis of K562 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
| Application Notes | WB~~1:100~500 IHC~~1:50~100 FC~~1:10~50: |
|---|
Additional Information
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
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