All products Antibodies Neprilysin Antibody (C-term)

Neprilysin Antibody (C-term)

Catalog Number：OM215196

  • Western blot analysis of Neprilysin Antibody (C-term) in A2058,A375,Ramos cell line lysates (35ug/lane). EPN (arrow) was detected using the purified Pab.

  • Formalin-fixed and paraffin-embedded human kidney carcinoma with Neprilysin Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

  • Neprilysin Antibody (C-term) flow cytometry analysis of Ramos cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Product Profile

Product Name Neprilysin Antibody (C-term)
Antibody Type Primary Antibodies

Key Feature

Clonality Polyclonal
Isotype Ig
Host Species Rabbit
Tested Applications

WBIHCFC
WB~~1:100~500 IHC~~1:50~100 FC~~1:10~50:
Species Reactivity

Human
Concentration 1 mg/ml
Purification

Target Information

Gene Synonyms
EPN
Alternative Names
MME
EPN
Neprilysin
Atriopeptidase
Common acute lymphocytic leukemia antigen
Enkephalinase
Neutral endopeptidase 24.11
Skin fibroblast elastase
CD_antigen=CD10
Molecular Weight(MW) 85514 Da
Function
Thermolysin-like specificity, but is almost confined on acting on polypeptides of up to 30 amino acids. Biologically important in the destruction of opioid peptides such as Met- and Leu-enkephalins by cleavage of a Gly-Phe bond. Able to cleave angiotensin-1, angiotensin-2 and angiotensin 1-9. Involved in the degradation of atrial natriuretic factor (ANF). Displays UV- inducible elastase activity toward skin preelastic and elastic fibers
Tissue Specificity This Neprilysin antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 513~542 amino acids from the C-terminal region of human Neprilysin.
Cellular Localization Cell membrane; Single-pass type II membrane protein

Database Links

Entrez Gene 4311

Application

  • Application

    Western blot analysis of Neprilysin Antibody (C-term) in A2058,A375,Ramos cell line lysates (35ug/lane). EPN (arrow) was detected using the purified Pab.

  • Application

    Formalin-fixed and paraffin-embedded human kidney carcinoma with Neprilysin Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

  • Application

    Neprilysin Antibody (C-term) flow cytometry analysis of Ramos cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Application Notes
WB~~1:100~500 IHC~~1:50~100 FC~~1:10~50:

Additional Information

Form Liquid
Storage Instructions For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Storage Buffer Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Note: The product is for research use only,not for use in diagnostic or therapeutic procedures.

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  • Catalog Number: OM215196
  • Price: $336.00
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  • Lead time: 2~3 Week

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