All products Antibodies RELA Antibody

RELA Antibody

Catalog Number：OM289755

  • Western blot analysis in Hela,K562,Raji,Ramos cell line lysates (35ug/lane).

  • Fluorescent confocal image of U251 cell stained with RELA Antibody .U251 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with RELA primary antibody (1:25). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin

Product Profile

Product Name RELA Antibody
Antibody Type Primary Antibodies
Product description 
NFKB1 (MIM 164011) or NFKB2 (MIM 164012) is bound to REL
(MIM 164910), RELA, or RELB (MIM 604758) to form the NFKB complex.
The p50 (NFKB1)/p65 (RELA) heterodimer is the most abundant form of
NFKB. The NFKB complex is inhibited by I-kappa-B proteins (NFKBIA,
MIM 164008 or NFKBIB, MIM 604495), which inactivate NFKB by
trapping it in the cytoplasm. Phosphorylation of serine residues on
the I-kappa-B proteins by kinases (IKBKA, MIM 600664, or IKBKB, MIM
603258) marks them for destruction via the ubiquitination pathway,
thereby allowing activation of the NFKB complex. Activated NFKB
complex translocates into the nucleus and binds DNA at
kappa-B-binding motifs such as 5-prime GGGRNNYYCC 3-prime or
5-prime HGGARNYYCC 3-prime (where H is A, C, or T; R is an A or G
purine; and Y is a C or T pyrimidine).1) Pan, W.W., et al. J. Biol. Chem. 285(45):34348-34354(2010)
Immunogen 
This RELA antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 166-195 amino acids from the Central region of human RELA.

Key Feature

Clonality Polyclonal
Isotype Ig
Host Species Rabbit
Tested Applications

IFWB
For IF starting dilution is: 1:10~50 For WB starting dilution is: 1:1000:
Species Reactivity

Human
Concentration 1 mg/ml
Purification Affinity purified

Target Information

Gene Symbol RELA
Alternative Names
Transcription factor p65
Nuclear factor NF-kappa-B p65 subunit
Nuclear factor of kappa light polypeptide gene enhancer in B-cells 3
RELA
NFKB3
Molecular Weight(MW) 60 kDa
Sequence Similarities Predicted species reactivity based on immunogen sequence: Mouse

Database Links

Entrez Gene 5970
Protein Accession Q04206

Application

  • Application

    Western blot analysis in Hela,K562,Raji,Ramos cell line lysates (35ug/lane).

  • Application

    Fluorescent confocal image of U251 cell stained with RELA Antibody .U251 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with RELA primary antibody (1:25). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin

Application Notes
For IF starting dilution is: 1:10~50 For WB starting dilution is: 1:1000:

Additional Information

Form Liquid
Storage Instructions Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Storage Buffer Supplied in PBS with 0.09% (W/V) sodium azide.
Note: The product is for research use only,not for use in diagnostic or therapeutic procedures.

Submit an review for OM289755
Submit an review for OM289755
  • Catalog Number: OM289755
  • Price: $336.00
  • Discount Price
  • Size:
  • Store: Out of stock
  • Lead time: 4~6 Week

  • Quantity:
    - +
  • Shipping to (Please select)
Add to cart
Add to Wishlist
Search the catalog number from any major antibody supplier/brand in the search bar above. The search engine will return OmnimAbs products for these targets.
PRODUCT CATEGORIES
Primary antibodies Secondary antibodies Tags antibodies Flow cytometry antibodies
SUPPORT
Catalogs Newsletter Order Online Buying FAQs Distributor
COMPANY
Profile Strategy Careers
CONTACT
Contact us

2013 © Omnimabs , All Rights Reserved.