Western blot analysis of RGS4 Antibody in MDA-MB231 cell line lysates (35ug/lane)
Western blot analysis of RGS4 Antibody in mouse brain tissue lysates (35ug/lane)
Western blot analysis of RGS4 using rabbit polyclonal RGS4 Antibody using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the RGS4 gene.
Regulator of G protein signaling (RGS) family members are regulatory molecules that act as GTPase activating proteins (GAPs) for G alpha subunits of heterotrimeric G proteins. RGS proteins are able to deactivate G protein subunits of the Gi alpha, Go alpha and Gq alpha subtypes. They drive G proteins into their inactive GDP-bound forms. Regulator of G protein signaling 4 belongs to this family. All RGS proteins share a conserved 120-amino acid sequence termed the RGS domain. Regulator of G protein signaling 4 protein is 37% identical to RGS1 and 97% identical to rat Rgs4. This protein negatively regulate signaling upstream or at the level of the heterotrimeric G protein and is localized in the cytoplasm.1) Heximer,S.P., et.al., Proc. Natl. Acad. Sci. U.S.A. 94 (26), 14389-14393 (1997)
Immunogen
This RGS4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 147-174 amino acids from the Central region of human RGS4.
Western blot analysis of RGS4 Antibody in MDA-MB231 cell line lysates (35ug/lane)
Application
Western blot analysis of RGS4 Antibody in mouse brain tissue lysates (35ug/lane)
Application
Western blot analysis of RGS4 using rabbit polyclonal RGS4 Antibody using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the RGS4 gene.
Application Notes
For WB starting dilution is: 1:1000:
Additional Information
Form
Liquid
Storage Instructions
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Storage Buffer
Supplied in PBS with 0.09% (W/V) sodium azide.
Note: The product is for research use only,not for use in diagnostic or therapeutic procedures.
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