StrepII-tag Antibody: Streptavidin is a tetrameric protein purified from Streptomyces avidinii. It has wide use in numerous molecular biological protocols dues to its strong affinity for biotin. The original Strep-tag is a nine amino acid peptide with high specificity and affinity towards streptavidin. The addition of this tag to the C-terminus of recombinant proteins allowed the simple purification of protein by use of affinity columns. It was re-engineered to allow it to also placed at the N-terminus of recombinant proteins.1) Schmidt TGM and Skerra A. The random peptide-assisted engineering of a C-terminal affinity peptide, useful for the detection and purification of a functional Ig Fv fragment. Protein Eng.1993; 6:109-22.
Immunogen
StrepII-tag antibody was raised against the amino acid sequence "asWSHPQFEKga".
Key Feature
Clonality
Polyclonal
Isotype
IgG
Host Species
Rabbit
Tested Applications
ELISAWB
StrepII-tag antibody can be used for the detection of StrepII-tagged proteins by Western blot at 1 μg/mL.:
Species Reactivity
Tag
Concentration
1 mg/ml
Purification
Affinity purified
Target Information
Alternative Names
StrepII-tag Antibody:
Application
Application
Western blot analysis of (A) 5 and (B) 25ng of StrepII-tagged recombinant protein with StrepII antibody at 1 μg/mL.
Application Notes
StrepII-tag antibody can be used for the detection of StrepII-tagged proteins by Western blot at 1 μg/mL.:
Additional Information
Form
Liquid
Storage Instructions
StrepII-tag antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Storage Buffer
StrepII-tag Antibody is supplied in PBS containing 0.02% sodium azide.
Note: The product is for research use only,not for use in diagnostic or therapeutic procedures.
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