Initiation of transcription by RNA polymerase II requires the activities of more than 70 polypeptides. The protein that coordinates these activities is transcription factor IID (TFIID), which binds to the core promoter to position the polymerase properly, serves as the scaffold for assembly of the remainder of the transcription complex, and acts as a channel for regulatory signals. TFIID is composed of the TATA-binding protein (TBP) and a group of evolutionarily conserved proteins known as TBP-associated factors or TAFs. TAFs may participate in basal transcription, serve as coactivators, function in promoter recognition or modify general transcription factors (GTFs) to facilitate complex assembly and transcription initiation. TAF15 encodes a subunit of TFIID present in a subset of TFIID complexes. Translocations involving chromosome 17 and chromosome 9, where the gene for the nuclear receptor CSMF is located, result in a gene fusion product that is an RNA binding protein associated with a subset of extraskeletal myxoid chondrosarcomas.1) Lee, H.J., et al., (2004) FEBS Lett. 564 (1-2), 188-198.
Immunogen
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human TAF15.
Key Feature
Clonality
Polyclonal
Host Species
Rabbit
Tested Applications
ELISAWB
TAF15 antibody can be used for detection of TAF15 by ELISA at 1:312500. TAF15 antibody can be used for detection of TAF15 by western blot at 3.0 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.:
TAF15 antibody can be used for detection of TAF15 by ELISA at 1:312500. TAF15 antibody can be used for detection of TAF15 by western blot at 3.0 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.:
Additional Information
Form
Liquid
Storage Instructions
For short periods of storage (days) store at 4˚C. For longer periods of storage, store TAF15 antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles.
Storage Buffer
IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt
Note: The product is for research use only,not for use in diagnostic or therapeutic procedures.
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