UBE1C

Catalog Number：OM638953

  • Fig1: Western blot analysis of UBE1C on human liver tissue lysate using anti-UBE1C antibody at 1/200 dilution.

  • Fig2: ICC staining UBE1C in A431 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

  • Fig3: ICC staining UBE1C in HUVEC cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

  • Fig4: ICC staining UBE1C in SH-SY5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

  • Fig5: Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-UBE1C antibody. Counter stained with hematoxylin.

  • Fig6: Immunohistochemical analysis of paraffin-embedded human pancreas tissue using anti-UBE1C antibody. Counter stained with hematoxylin.

  • Fig7: Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti-UBE1C antibody. Counter stained with hematoxylin.

  • Fig8: Flow cytometric analysis of HUVEC cells with UBE1C antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; green).

Product Profile

Product Name UBE1C
Antibody Type Primary Antibodies
Product description 
Ubiquitin is an abundant, highly conserved protein found in all eukaryotic cells either free or covalently attached to cellular proteins. The primary function of ubiquitin in mammalian systems is to clear abnormal, foreign and improperly folded proteins by targeting them for proteosome degradation. UBA3 (ubiquitin-like modifier activating enzyme 3), also known as NEDD8-activating enzyme E1 catalytic subunit or UBE1C (ubiquitin-activating enzyme E1C), is a 463 amino acid protein belonging to the ubiquitin-activating E1 family and UBA3 subfamily. Ubiquitously expressed, UBA3 acts as an activator to NEDD8, a ubiquitin-like protein, thus regulating cell division, signaling and embryogenesis. UBA3 exists as two isoforms due to alternative splicing events.
Immunogen 
Recombinant protein

Key Feature

Clonality Polyclonal
Isotype IgG
Host Species Rabbit
Tested Applications

WBICCIHCFC
WB:1:500
ICC:1:50-1:200
IHC:1:50-1:200
FC:1:50-1:100
Species Reactivity

HumanMouseRat
Concentration 1 mg/mL.

Target Information

Alternative Names
DKFZp566J164 antibody EC 6.3.2. antibody hUba3 antibody MGC22384 antibody NEDD8 activating enzyme E1 catalytic subunit antibody NEDD8 activating enzyme E1C antibody Nedd8 activating enzyme hUba3 antibody NEDD8-activating enzyme E1 catalytic subunit antibody NEDD8-activating enzyme E1C antibody uba3 antibody UBA3 ubiquitin activating enzyme E1 homolog antibody UBA3_HUMAN antibody UBE1C antibody Ubiquitin activating enzyme 3 antibody Ubiquitin activating enzyme E1C antibody Ubiquitin-activating enzyme 3 antibody Ubiquitin-activating enzyme E1C antibody Ubiquitin-like modifier-activating enzyme 3 antibody
Molecular Weight(MW) 52 kDa
Cellular Localization Nucleus. Cytoplasm.

Database Links

SwissProt ID Q8TBC4
Q8C878
Q99MI7

Application

  • Application

    Fig1: Western blot analysis of UBE1C on human liver tissue lysate using anti-UBE1C antibody at 1/200 dilution.

  • Application

    Fig2: ICC staining UBE1C in A431 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

  • Application

    Fig3: ICC staining UBE1C in HUVEC cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

  • Application

    Fig4: ICC staining UBE1C in SH-SY5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

  • Application

    Fig5: Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-UBE1C antibody. Counter stained with hematoxylin.

  • Application

    Fig6: Immunohistochemical analysis of paraffin-embedded human pancreas tissue using anti-UBE1C antibody. Counter stained with hematoxylin.

  • Application

    Fig7: Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti-UBE1C antibody. Counter stained with hematoxylin.

  • Application

    Fig8: Flow cytometric analysis of HUVEC cells with UBE1C antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; green).

Positive Control Human liver tissue lysate, A431, HUVEC, SH-SY5Y, rat brain tissue, human pancreas tissue, mouse testis tissue.
Application Notes
WB:1:500
ICC:1:50-1:200
IHC:1:50-1:200
FC:1:50-1:100

Additional Information

Form Liquid
Storage Instructions Store at +4℃after thawing. Aliquot store at -20℃or -80℃Avoid repeated freeze / thaw cycles.
Storage Buffer 1*TBS (pH7.4), 0.5%BSA, 50%Glycerol. Preservative: 0.05% Sodium Azide.
Note: The product is for research use only,not for use in diagnostic or therapeutic procedures.

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  • Catalog Number: OM638953
  • Price: $336.00
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  • Lead time: 4~6 Week

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