Lanes:1:non-transfected cells; 2:V5-tagged empty plasmid; 3:protein A (V5-tagged); 4:protein B (V5-tagged); 5:protein C (V5-tagged); 6:protein D (V5-tagged); Protocol and data courtesy of Dr. Gustavo Gutierrez, Burnham Institute for Medical Research.
Western blot analysis of anti-V5 Tag Antibody in 12tag protein (35ug/lane). 12 Tag (arrow) was detected using the purified Pab.
KLH conjugated synthetic peptide encoding the V5 tag (GKPIPNPLLGLDST) was used as antigen.
Application
Application
Lanes:1:non-transfected cells; 2:V5-tagged empty plasmid; 3:protein A (V5-tagged); 4:protein B (V5-tagged); 5:protein C (V5-tagged); 6:protein D (V5-tagged); Protocol and data courtesy of Dr. Gustavo Gutierrez, Burnham Institute for Medical Research.
Application
Western blot analysis of anti-V5 Tag Antibody in 12tag protein (35ug/lane). 12 Tag (arrow) was detected using the purified Pab.
Application Notes
WB~~1:100~500:
Additional Information
Form
Liquid
Storage Instructions
For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Storage Buffer
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
Note: The product is for research use only,not for use in diagnostic or therapeutic procedures.
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