WDR5

Catalog Number：OM638924

  • Fig1: Western blot analysis of WDR5 on MCF-7 cell lysate using anti-WDR5 antibody at 1/500 dilution.

  • Fig2: ICC staining WDR5 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

  • Fig3: ICC staining WDR5 in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

  • Fig4: ICC staining WDR5 in PC-3M cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

  • Fig5: Flow cytometric analysis of MCF-7 cells with WDR5 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.

Product Profile

Product Name WDR5
Antibody Type Primary Antibodies
Product description 
WD-repeat protein 5 (WDR5, also designated BMP-2-induced gene 3 kb or BIG-3) belongs to the family of WD-40 repeat proteins, and is essential for vertebrate development, Hox gene activation and global H3K4 trimethylation. WDR5 is a conserved subunit of Trithorax (TRX) histone methyltransferase complexes that selectively binds to dimethylated Lys4 (K4me2) in histone H3 to promote K4 trimethylation by TRX. It is expressed in osteoblasts, chondrocytes, osteocytes and marrow stromal cells. The WDR5 protein contains 7 WD-repeats, which may play a role in its function of accelerating osteoblast differentiation.
Immunogen 
Recombinant protein

Key Feature

Clonality Polyclonal
Isotype IgG
Host Species Rabbit
Tested Applications

WBICCFC
WB:1:500-1:1,000
ICC:1:50-1:200
FC:1:50-1:100
Species Reactivity

Human
Concentration 1 mg/mL.

Target Information

Alternative Names
2410008O07Rik antibody AA408785 antibody AA960360 antibody BIG 3 antibody Big antibody BIG3 antibody BMP2 induced 3 kb gene protein antibody BMP2 induced gene
3-KB antibody BMP2-induced 3-kb gene protein antibody MGC114572 antibody OTTHUMP00000162494 antibody SWD 3 antibody SWD3 antibody SWD3 Set1c WD40 repeat protein homolog antibody WD repeat containing protein 5 antibody WD repeat domain 5 antibody WD repeat protein 5 antibody WD repeat-containing protein 5 antibody WD repeat-containing protein BIG-3 antibody WDR 5 antibody Wdr5 antibody WDR5_HUMAN antibody
Molecular Weight(MW) 36 kDa, additional band 70 kDa.
Cellular Localization Nucleus.

Database Links

SwissProt ID P61964

Application

  • Application

    Fig1: Western blot analysis of WDR5 on MCF-7 cell lysate using anti-WDR5 antibody at 1/500 dilution.

  • Application

    Fig2: ICC staining WDR5 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

  • Application

    Fig3: ICC staining WDR5 in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

  • Application

    Fig4: ICC staining WDR5 in PC-3M cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

  • Application

    Fig5: Flow cytometric analysis of MCF-7 cells with WDR5 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.

Positive Control Hela, MCF-7, PC-3M.
Application Notes
WB:1:500-1:1,000
ICC:1:50-1:200
FC:1:50-1:100

Additional Information

Form Liquid
Storage Instructions Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
Storage Buffer 1*TBS (pH7.4), 0.5%BSA, 50%Glycerol. Preservative: 0.05% Sodium Azide.
Note: The product is for research use only,not for use in diagnostic or therapeutic procedures.

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  • Catalog Number: OM638924
  • Price: $336.00
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  • Lead time: 4~6 Week

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