Fig1: Western blot analysis of eIF-4E on different cell lysates using anti-eIF-4E antibody at 1/500 dilution. Positive control: Lane1: PC-12 Lane2: NIH-3T3
Fig2: ICC staining eIF-4E in A431 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Fig3: ICC staining eIF-4E in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Fig4: ICC staining eIF-4E in SK-Br-3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Fig5: Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-eIF-4E antibody. Counter stained with hematoxylin.
Fig6: Immunohistochemical analysis of paraffin-embedded mouse colon tissue using anti-eIF-4E antibody. Counter stained with hematoxylin.
Fig7: Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-eIF-4E antibody. Counter stained with hematoxylin.
Fig8: Immunohistochemical analysis of paraffin-embedded mouse hippocampus tissue using anti-eIF-4E antibody. Counter stained with hematoxylin.
Fig9: Flow cytometric analysis of 293T cells with eIF-4E antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black).
Product Name | eIF-4E |
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Antibody Type | Primary Antibodies |
Product description |
|
Immunogen | Peptide |
Clonality | Polyclonal |
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Isotype | IgG |
Host Species | Rabbit |
Tested Applications | |
WB:1:500-1:1,000 ICC:1:50-1:200 IHC:1:50-1:100 FC:1:50-1:100 |
|
Species Reactivity | |
Concentration | 1 mg/mL. |
Alternative Names | AUTS19 antibody
CBP antibody
eIF 4E antibody
eIF 4F 25 kDa subunit antibody
EIF 4F antibody
eIF-4E antibody
eIF-4F 25 kDa subunit antibody
eIF4E antibody
EIF4E1 antibody
EIF4EL1 antibody
EIF4F antibody
Eukaryotic translation initiation factor 4 E antibody
Eukaryotic translation initiation factor 4E antibody
Eukaryotic translation initiation factor 4E like 1 antibody
IF4E_HUMAN antibody
Messanger RNA Cap Binding Protein eIF 4E antibody
MGC111573 antibody
mRNA cap binding protein antibody
mRNA cap-binding protein antibody
|
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Molecular Weight(MW) | 25 kDa |
Cellular Localization | Cytoplasm. |
SwissProt ID | P06730 P63073 P63074 |
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Fig1: Western blot analysis of eIF-4E on different cell lysates using anti-eIF-4E antibody at 1/500 dilution. Positive control: Lane1: PC-12 Lane2: NIH-3T3
Fig2: ICC staining eIF-4E in A431 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Fig3: ICC staining eIF-4E in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Fig4: ICC staining eIF-4E in SK-Br-3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Fig5: Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-eIF-4E antibody. Counter stained with hematoxylin.
Fig6: Immunohistochemical analysis of paraffin-embedded mouse colon tissue using anti-eIF-4E antibody. Counter stained with hematoxylin.
Fig7: Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-eIF-4E antibody. Counter stained with hematoxylin.
Fig8: Immunohistochemical analysis of paraffin-embedded mouse hippocampus tissue using anti-eIF-4E antibody. Counter stained with hematoxylin.
Fig9: Flow cytometric analysis of 293T cells with eIF-4E antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black).
Positive Control | PC-12, NIH-3T3, A431, Hela, SK-Br-3, 293T, human colon cancer tissue, mouse colon tissue, mouse brain tissue, mouse hippocampus tissue. |
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Application Notes | WB:1:500-1:1,000 ICC:1:50-1:200 IHC:1:50-1:100 FC:1:50-1:100 |
Form | Liquid |
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Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
Storage Buffer | 1*TBS (pH7.4), 0.5%BSA, 50%Glycerol. Preservative: 0.05% Sodium Azide. |
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