Immunohistochemistry
Immunohistochemistry with Human Tonsil lysate tissue at an antibody concentration of 5.0ug/ml using anti-NCOA3 antibodyWestern blot
Host: Rabbit Target Name: NCOA3 Sample Tissue: MCF7 Antibody Dilution: 1.0ug/mlWestern blot
0.2-1 ug/mlELISA Titer: 1:312500Positive Control: 293T cell lysate| Product Name | Rabbit anti-NCOA3 polyclonal antibody - middle region |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | ACTR, AIB-1, AIB1, CAGH16, CTG26, KAT13B, MGC141848, RAC3, SRC-1, SRC3, TNRC14, TNRC16, TRAM-1, pCIP, SRC-3, bHLHe42 |
| Immunogen | The immunogen for anti-NCOA3 antibody: synthetic peptide directed towards the middle region of human NCOA3 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIHC |
| WB:1:500~1:2000 IHC:1:50~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | BovineHorseHumanPig |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | NCOA3 |
|---|---|
| Gene Synonyms | ACTR AIB-1 AIB1 CAGH16 CTG26 KAT13B MGC141848 RAC3 SRC-1 SRC3 TNRC14 TNRC16 TRAM-1 pCIP SRC-3 bHLHe42 |
| Gene Full Name | Nuclear receptor coactivator 3 |
| Gene Summary | NCOA3 is a nuclear receptor coactivator that interacts with nuclear hormone receptors to enhance their transcriptional activator functions. The encoded protein has histone acetyltransferase activity and recruits p300/CBP-associated factor and CREB binding protein as part of a multisubunit coactivation complex. This protein is initially found in the cytoplasm but is translocated into the nucleus upon phosphorylation. Two transcript variants encoding different isoforms have been found for this gene. In addition, a polymorphic repeat region is found in the C-terminus of the encoded protein.The protein encoded by this gene is a nuclear receptor coactivator that interacts with nuclear hormone receptors to enhance their transcriptional activator functions. The encoded protein has histone acetyltransferase activity and recruits p300/CBP-associated factor and CREB binding protein as part of a multisubunit coactivation complex. This protein is initially found in the cytoplasm but is translocated into the nucleus upon phosphorylation. Two transcript variants encoding different isoforms have been found for this gene. In addition, a polymorphic repeat region is found in the C-terminus of the encoded protein. |
| Alternative Names | ACTR AIB-1 AIB1 CAGH16 CTG26 KAT13B MGC141848 RAC3 SRC-1 SRC3 TNRC14 TNRC16 TRAM-1 pCIP SRC-3 bHLHe42 |
| Molecular Weight(MW) | 155kDa |
| Sequence | 1420 amino acids |
Immunohistochemistry
Immunohistochemistry with Human Tonsil lysate tissue at an antibody concentration of 5.0ug/ml using anti-NCOA3 antibody
Western blot
Host: Rabbit Target Name: NCOA3 Sample Tissue: MCF7 Antibody Dilution: 1.0ug/ml
Western blot
0.2-1 ug/mlELISA Titer: 1:312500Positive Control: 293T cell lysate| Application Notes | WB:1:500~1:2000 IHC:1:50~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Aliquot and store at -20°C. Avoid repeated freeze / thaw cycles |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
