WB
Various whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-NCoA 3 antibody. The HRP-conjugated Goat anti-Rabbit IgG (H + L) antibody was used to detect the antibody.IHC
Human salivary gland was stained with anti-NCoA 3 Rabbit antibody.| Product Name | NCoA 3 Rabbit mAb |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | The specific immunogen used to produce this antibody is proprietary information. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG,K |
| Host Species | Rabbit |
| Tested Applications | IHCWB |
| IHC:1:200-1:500 WB:1:2000-1:10000 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | NCOA3 |
|---|---|
| Gene Synonyms | ACTR AIB1 RAC3 SRC3 pCIP AIB-1 CTG26 SRC-3 CAGH16 KAT13B TNRC14 TNRC16 TRAM-1 bHLHe42 |
| Gene Full Name | nuclear receptor coactivator 3 |
| Gene Summary | The protein encoded by this gene is a nuclear receptor coactivator that interacts with nuclear hormone receptors to enhance their transcriptional activator functions. The encoded protein has histone acetyltransferase activity and recruits p300/CBP-associated factor and CREB binding protein as part of a multisubunit coactivation complex. This protein is initially found in the cytoplasm but is translocated into the nucleus upon phosphorylation. Several transcript variants encoding different isoforms have been found for this gene. In addition, a polymorphic repeat region is found in the C-terminus of the encoded protein. [provided by RefSeq, Mar 2010] |
| Alternative Names | NCOA3 AIB1 BHLHE42 RAC3 TRAM1 Nuclear receptor coactivator 3 NCoA-3 ACTR Amplified in breast cancer 1 protein AIB-1 CBP-interacting protein pCIP Class E basic helix-loop-helix protein 42 bHLHe42 Receptor-associated coactivator 3 RAC-3 Steroid receptor coactivator protein 3 SRC-3 Thyroid hormone receptor activator molecule 1 TRAM-1 |
| Molecular Weight(MW) | 155 kDa (Observed band size: 160kDa) |
| Cellular Localization | Cytoplasm. Nucleus. Mainly cytoplasmic and weakly nuclear. Upon TNF activation and subsequent phosphorylation , it translocates from the cytoplasm to the nucleus. |
WB
Various whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-NCoA 3 antibody. The HRP-conjugated Goat anti-Rabbit IgG (H + L) antibody was used to detect the antibody.
IHC
Human salivary gland was stained with anti-NCoA 3 Rabbit antibody.| Application Notes | IHC:1:200-1:500 WB:1:2000-1:10000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | -15°C to -25°C/1 year(Do not lower than -25°C) |
| Storage Buffer | PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA |
