WB
Western blot analysis of extracts from HeLa cells using SRC3 Rabbit mAb at 1:1000.ICC/IF
Immunofluorescence analysis of HeLa cells labelling SRC3 with SRC3 Rabbit mAb. The cells were fixed with 4% PFA (10min, RT) followed by treatment with 0.1% Triton X-100 (10min, RT), and blocked in 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween 20 for 1h. The cells were then incubate with SRC3 Rabbit mAb (1:100) at room temprature for 1h, followed by a further incubation at room temperature for 45min with Goat Anti Rabbit lgG (H+L)-AF488 (shown in green). Nuclear DNA was labeled in blue with DAPI. Control: Secondary antibody only.| Product Name | SRC3 Rabbit mAb |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | A synthetic peptide of human SRC3. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFWB |
| WB:1:1000-1:5000 ICC/IF:1:50-1:100 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | NCOA3 |
|---|---|
| Gene Synonyms | ACTR AIB1 RAC3 SRC3 pCIP AIB-1 CTG26 SRC-3 CAGH16 KAT13B TNRC14 TNRC16 TRAM-1 bHLHe42 |
| Gene Full Name | nuclear receptor coactivator 3 |
| Gene Summary | The protein encoded by this gene is a nuclear receptor coactivator that interacts with nuclear hormone receptors to enhance their transcriptional activator functions. The encoded protein has histone acetyltransferase activity and recruits p300/CBP-associated factor and CREB binding protein as part of a multisubunit coactivation complex. This protein is initially found in the cytoplasm but is translocated into the nucleus upon phosphorylation. Several transcript variants encoding different isoforms have been found for this gene. In addition, a polymorphic repeat region is found in the C-terminus of the encoded protein. [provided by RefSeq, Mar 2010] |
| Alternative Names | ACTR AIB1 RAC3 SRC3 pCIP AIB-1 CTG26 SRC-3 CAGH16 KAT13B TNRC14 TNRC16 TRAM-1 bHLHe42 |
| Molecular Weight(MW) | 155 kDa(Observed band size: 160 kDa) |
| Cellular Localization | Cytoplasm,Nucleus. |
| SwissProt ID | Q9Y6Q9 |
|---|
WB
Western blot analysis of extracts from HeLa cells using SRC3 Rabbit mAb at 1:1000.
ICC/IF
Immunofluorescence analysis of HeLa cells labelling SRC3 with SRC3 Rabbit mAb. The cells were fixed with 4% PFA (10min, RT) followed by treatment with 0.1% Triton X-100 (10min, RT), and blocked in 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween 20 for 1h. The cells were then incubate with SRC3 Rabbit mAb (1:100) at room temprature for 1h, followed by a further incubation at room temperature for 45min with Goat Anti Rabbit lgG (H+L)-AF488 (shown in green). Nuclear DNA was labeled in blue with DAPI. Control: Secondary antibody only.| Application Notes | WB:1:1000-1:5000 ICC/IF:1:50-1:100 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20°C.Stable for 12 months from date of receipt. |
| Storage Buffer | Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA. |
