Western blot
Sample: 1. DT40 (5x10
5cells)
2. DT40 + rec. hTDP2 (5x10
5cells)
3. soluble HIS-hTDP2 (5x10
5cells)
4. pellet HIS-hTDP2 (5x10
5cells)
Primary dilution:1:1000
Secondary Antibody: dakocytomation Goat anti-rabbit
Secondary dilution: 1:5000
Image Submitted by: Keith Caldecott
University of Sussex
Western blot
0.2-1 ug/ml
Positive Control: HepG2 cell lysate