The TTRAP gene encodes a member of a superfamily of divalent cation-dependent phosphodiesterases. The encoded protein associates with CD40, tumor necrosis factor (TNF) receptor-75 and TNF receptor associated factors (TRAFs), and inhibits nuclear factor-kappa-B activation. This protein has sequence and structural similarities with APE1 endonuclease, which is involved in both DNA repair and the activation of transcription factors.1) Pei, H., et al., (2003) Oncogene 22 (18), 2699-2709.
Immunogen
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human TTRAP.
Key Feature
Clonality
Polyclonal
Host Species
Rabbit
Tested Applications
ELISAIHCWB
TTRAP antibody can be used for detection of TTRAP by ELISA at 1:62500. TTRAP antibody can be used for detection of TTRAP by western blot at 0.5 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.:
Antibody used in WB on Human HepG2 at 0.2-1 ug/ml.
Application
Antibody used in WB on r-tdp2 at 1:1000.
Positive Control
1) Cat. No. 1211 - HepG2 Cell Lysate
Application Notes
TTRAP antibody can be used for detection of TTRAP by ELISA at 1:62500. TTRAP antibody can be used for detection of TTRAP by western blot at 0.5 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.:
Additional Information
Form
Liquid
Storage Instructions
For short periods of storage (days) store at 4˚C. For longer periods of storage, store TTRAP antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles.
Storage Buffer
Antibody is lyophilized in PBS buffer with 2% sucrose. Add 50 μL of distilled water. Final antibody concentration is 1 mg/mL.