Application
Flow cytometric analysis of A549 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.Application
Confocal immunofluorescent analysis of EAPII Antibody with A549 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red). DAPI was used to stain the cell nuclear (blue).Application
EAPII Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human prostate carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.Application
Western blot analysis in A549 cell line lysates (35ug/lane).| Product Name | EAPII Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Tyrosyl-DNA phosphodiesterase 2, Tyr-DNA phosphodiesterase 2, hTDP2, 314-, 5'-tyrosyl-DNA phosphodiesterase, 5'-Tyr-DNA phosphodiesterase, ETS1-associated protein 2, ETS1-associated protein II, EAPII, TRAF and TNF receptor-associated protein, Tyrosyl-RNA p |
| Product description | This gene encodes a member of a superfamily of divalent cation-dependent phosphodiesterases. The encoded protein associates with CD40, tumor necrosis factor (TNF) receptor-75 and TNF receptor associated factors (TRAFs), and inhibits nuclear factor-kappa-B activation. This protein has sequence and structural similarities with APE1 endonuclease, which is involved in both DNA repair and the activation of transcription factors.1) Shimada, M., et al. Hum. Genet. 128(4):433-441(2010) |
| Immunogen | This EAPII antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 246-272 amino acids from the C-terminal region of human EAPII. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | FACSIFIHC-PWB |
| For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:10~50 For IF starting dilution is: 1:10~50 For FACS starting dilution is: 1:10~50: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | TDP2 |
|---|---|
| Alternative Names | Tyrosyl-DNA phosphodiesterase 2 Tyr-DNA phosphodiesterase 2 hTDP2 314- 5'-tyrosyl-DNA phosphodiesterase 5'-Tyr-DNA phosphodiesterase ETS1-associated protein 2 ETS1-associated protein II EAPII TRAF and TNF receptor-associated protein Tyrosyl-RNA p |
| Molecular Weight(MW) | 41 kDa |
Application
Flow cytometric analysis of A549 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Application
Confocal immunofluorescent analysis of EAPII Antibody with A549 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red). DAPI was used to stain the cell nuclear (blue).
Application
EAPII Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human prostate carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.
Application
Western blot analysis in A549 cell line lysates (35ug/lane).| Application Notes | For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:10~50 For IF starting dilution is: 1:10~50 For FACS starting dilution is: 1:10~50: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
