Immunoblot
Rat brain sectionPrimary Antibody Dilution: 1:500Secondary Antibody: Anti-rabbit-biotin, streptavidin-diaminobenzidineSecondary Antibody Dilution: 1:500Gene Name: KCNN2Submitted by: Dr. Amiel Rosenkranz, Rosalind Franklin UniversityWestern blot
Host: Rabbit Target Name:KCNN2 Sample Tissue:HepG2Lane A: Primary Antibody Lane B: Primary Antibody + Blocking Peptide Primary Antibody Concentration:2.5ug/mL Peptide Concentration: 2.0ug/mL Lysate Quantity: 25ug/lane Gel Concentration: 12%| Product Name | Rabbit anti-KCNN2 polyclonal antibody - C-terminal region |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | SK2, hSK2, SKCA2, KCa2.2 |
| Immunogen | The immunogen for anti-KCNN2 antibody: synthetic peptide directed towards the C terminal of human KCNN2 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | BovineDogHorseHumanMousePigRabbitRat |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | KCNN2 |
|---|---|
| Gene Synonyms | SK2 hSK2 SKCA2 KCa2.2 |
| Gene Full Name | Potassium intermediate/small conductance calcium-activated channel, subfamily N, member 2 |
| Gene Summary | Action potentials in vertebrate neurons are followed by an afterhyperpolarization (AHP) that may persist for several seconds and may have profound consequences for the firing pattern of the neuron. Each component of the AHP is kinetically distinct and is mediated by different calcium-activated potassium channels. The protein encoded by KCNN2 is activated before membrane hyperpolarization and is thought to regulate neuronal excitability by contributing to the slow component of synaptic AHP. The encoded protein is an integral membrane protein that forms a voltage-independent calcium-activated channel with three other calmodulin-binding subunits. KCNN2 is a member of the KCNN family of potassium channel genes. |
| Alternative Names | SK2 hSK2 SKCA2 KCa2.2 |
| Molecular Weight(MW) | 64kDa |
| Sequence | 579 amino acids |
Immunoblot
Rat brain sectionPrimary Antibody Dilution: 1:500Secondary Antibody: Anti-rabbit-biotin, streptavidin-diaminobenzidineSecondary Antibody Dilution: 1:500Gene Name: KCNN2Submitted by: Dr. Amiel Rosenkranz, Rosalind Franklin University
Western blot
Host: Rabbit Target Name:KCNN2 Sample Tissue:HepG2Lane A: Primary Antibody Lane B: Primary Antibody + Blocking Peptide Primary Antibody Concentration:2.5ug/mL Peptide Concentration: 2.0ug/mL Lysate Quantity: 25ug/lane Gel Concentration: 12%| Application Notes | WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Aliquot and store at -20°C. Avoid repeated freeze / thaw cycles |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
