WB
Equivalent amounts of cell lysates (10 ug per lane) of wild-type HeLa cells (WT) and PAPSS2-Knockout HeLa cells (KO) were separated by SDS-PAGE and immunoblotted with anti-PAPSS2 monoclonal antibody (1:500`). Then the blotted membrane was stripped and reprobed with anti-HSP90 antibody as a loading control.IHC
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-PAPSS2 mouse monoclonal antibody. Heat-induced epitope retrieval by EDTA solution buffer pH 8.0 at 120°C for 3 min.IHC
Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-PAPSS2 mouse monoclonal antibody. Heat-induced epitope retrieval by EDTA solution buffer pH 8.0 at 120°C for 3 min.IHC
Immunohistochemical staining of paraffin-embedded Human Ovary tissue within the normal limits using anti-PAPSS2 mouse monoclonal antibody. Heat-induced epitope retrieval by EDTA solution buffer pH 8.0 at 120°C for 3 min.IHC
Immunohistochemical staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-PAPSS2 mouse monoclonal antibody. Heat-induced epitope retrieval by EDTA solution buffer pH 8.0 at 120°C for 3 min.IHC
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-PAPSS2 mouse monoclonal antibody. Heat-induced epitope retrieval by EDTA solution buffer pH 8.0 at 120°C for 3 min.| Product Name | PAPSS2 Mouse Monoclonal Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Human recombinant protein fragment corresponding to amino acids 1-247 of human PAPSS2(NP_004661) produced in E.coli. |
| Clonality | monoclonal |
|---|---|
| Isotype | IgG1 |
| Host Species | Mouse |
| Tested Applications | IHCWB |
| IHC:1:150 WB:1:200-1:2000 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Protein A/G |
| Gene Symbol | PAPSS2 |
|---|---|
| Gene Synonyms | SK2 BCYM4 ATPSK2 |
| Gene Full Name | 3'-phosphoadenosine 5'-phosphosulfate synthase 2 |
| Gene Summary | Sulfation is a common modification of endogenous (lipids, proteins, and carbohydrates) and exogenous (xenobiotics and drugs) compounds. In mammals, the sulfate source is 3'-phosphoadenosine 5'-phosphosulfate (PAPS), created from ATP and inorganic sulfate. Two different tissue isoforms encoded by different genes synthesize PAPS. This gene encodes one of the two PAPS synthetases. Defects in this gene cause the Pakistani type of spondyloepimetaphyseal dysplasia. Two alternatively spliced transcript variants that encode different isoforms have been described for this gene. [provided by RefSeq, Jul 2008] |
| Alternative Names | ATPSK2 BCYM4 SK2 |
| Molecular Weight(MW) | 69.3 kDa |
| Cellular Localization | cytosol,nucleus |
WB
Equivalent amounts of cell lysates (10 ug per lane) of wild-type HeLa cells (WT) and PAPSS2-Knockout HeLa cells (KO) were separated by SDS-PAGE and immunoblotted with anti-PAPSS2 monoclonal antibody (1:500`). Then the blotted membrane was stripped and reprobed with anti-HSP90 antibody as a loading control.
IHC
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-PAPSS2 mouse monoclonal antibody. Heat-induced epitope retrieval by EDTA solution buffer pH 8.0 at 120°C for 3 min.
IHC
Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-PAPSS2 mouse monoclonal antibody. Heat-induced epitope retrieval by EDTA solution buffer pH 8.0 at 120°C for 3 min.
IHC
Immunohistochemical staining of paraffin-embedded Human Ovary tissue within the normal limits using anti-PAPSS2 mouse monoclonal antibody. Heat-induced epitope retrieval by EDTA solution buffer pH 8.0 at 120°C for 3 min.
IHC
Immunohistochemical staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-PAPSS2 mouse monoclonal antibody. Heat-induced epitope retrieval by EDTA solution buffer pH 8.0 at 120°C for 3 min.
IHC
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-PAPSS2 mouse monoclonal antibody. Heat-induced epitope retrieval by EDTA solution buffer pH 8.0 at 120°C for 3 min.| Application Notes | IHC:1:150 WB:1:200-1:2000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20°C as received. |
| Storage Buffer | PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide. |
